Experimental Study On The Effect Of Buzhong Yiqi Decoction On The Apoptosis Of A549/DDP Cells Induced By Epithelial-mesenchymal Transition

Purpose:To explore the inhibitory effect of Buzhong Yiqi Decoction medicated on epithelial mesenchymal transition of lung adenocarcinoma A549/DDP cells.Material and method:Acording to the Buzhong Yiqi Decoction(astragalus me mbranaceus 18 g,licorice 9g,ginseng 6g,Chinese angelica 3g,orange peel 6g,cohosh 6g,radix bupleuri 6g,atractylodes 9g)will be bought them from liaoning university of trad itional Chinese medicine hospital.Buy rats from pharmaceutical companies and raise th em fill in yiqi decoction.According to the human body surface area tto feed the rats e very day.On the eighth day,we remove the mouse to another houser and extract ser um.These actions are for the subsequent tests.We cultured cells The resistance index of A549/DDP cells to cisplatin was measured by tetrazolium(MTT)assay.The cells w as cultured and divided into three groups: D(A549/DDP + blank serum)group,DT(A549/DDP + TGF-?1 + blank serum)group,DTB(A549/DDP + TGF-?1 + the best co ntaining medicine serum)group.The test of morpgology changes Cells A549/DDP medi ated by TGF-?1 was by cytomorphology The protein expression levels of Fas and Fas L were analyzed by meth of immunocytochemistry and Western blotting.To measure cells apoptosis was used by deoxyribose situ terminal transferase labeling(TUNEL)an d DAPI methed.We don't forget to sort out the data.Detection data are calculated by SPSS17.0 software.Statistical software for statistical analysis,data expression for the mean standard deviation(?x�S).The means differences between groups if meet the no rmality of variance,the comparison between groups will be use single factor analysi s of variance(ANOVA).The difference was statistically significant by P<0.05.Results: 1.The A549 / DDP cells resistant of DDP in this experiment.2.The TGF-?1 induced A549 / DDP cells epithelial mesenchymal typical in this experi ment.3.Reduced of apoptosis protein Fas,Fas L expression after A549 / DDP cell epithelial mesenchymal transformation.4.Early apoptosis cells decreases after A549 / DDP cells epithelial mesenchymal trans formation.5.The disappear of A549 / DDP cells into the typical epithelial mesenchymal transfor med by decoction.6.Fas and Fas L proteins expression by repair of decoction intervention A549 / DDP cells into epithelial mesenchymal transformation.7.Filled in yiqi decoction intervene early after A549 / DDP cells epithelial mesenchym al transformation and increased the number of apoptotic cells.Conclusion: 1.TGF-?1 can be induced A549 / DDP epithelial mesenchymal cell transformation.2.Buzhong Yiqi Decoction can influence the Fas/Fas L signal transduction pathway and promote the expression levels of Fas and Fas L,which inhibit the proliferation and induce apoptosis on A549/DDP cells.It may be related to the inhibition of the EMT process..