Functionalized Optoelectronic Nanomaterials Are Used For Tumor Marker Detection And In Situ Analysis Of Tumor Cells

Cancer is a major disease that threats human life,which also has a high fatality rate and moreand higher.Therefore,it is urgent to dedicate to the task of cancer treatment and prevention.For cancer,if you can early diagnosis and found or if we can take corresponding measures,the cure rate and survival rate of cancer patients has a lot to do.But now the tumor detection specificity and sensitivity is very low,the early detection of great difficulties.It is easy to delay the optimal timing of treatment for the slower conformed.In order to improve the specificity and sensitivity of cancer diagnosis method,we are dedicated to the detection of tumor markers and tumor cell in situ to realize the detection of cancer.In this paper,based on tumor markers such as AFP,miR-21,using photoelectrochemical method,realized the analysis of tumor markers and In situ analysis of tumor cells.The main works are listed below:1.We developed an efficient dual-quenching ECL immunosensor based on the novel ternary composite with high ECL intensity coupled with H2O2-related enzymatic reactions and ECL-RET strategy fromAuNRs.This ultrasensitive ECL sensing strategy combined the advantages of the excellent ECL sensing properties of graphene/Au-CdS:Eu QDs,and dual-quenching effect and achieve sensitive detection of alpha fetoprotein(AFP).2.We designed a new ternary composite(H-RGO-Au NFs)which showed excellent artificial enzymeproperties.Therefore,based on this new ma-terial and the aptamer technology,a smart electrochemical aptamerbiosensorfor K562cells(leukemia cancer cells)was further presented,with good performance in selectivity and sensitivity attributed to the excellent peroxidase-mimetic cata-lytic activity of H-RGO-Au NFs.3.We reported a strategy for the fabrication of Au NPs based fluorescence nanoprobe on which the probe DNA could bind target miRNA and arise following DNAzyme assistant DNA strand breakage,resulting in the fluorescein Cy5 released and away from Au NPs.This long-distance greatly weakens the quenching effect from Au NPs to the fluorescence of Cy5 and then Cy5 shows its fluorescence signal.That means one target-miR-21 binding could result in a couple of times of shear action which is effective for signal amplification.Because of the overexpression of miR-21 in cancer cells,this miRNA-responsive nanoscissor model paves a simple but effective way to distinguish cancer cells from normal cells and could further evaluate other important genes during intracellular amplified detecting and imaging.