High-density Lipoprotein Cholesterol Levels In Hypertensive Heart And Kidney Damage-specific Serum Proteins Are Associated With All-cause Mortality In Patients With Cerebral Thrombosis

Objective:Hypertension was one of the primary risk factors for cardiovascular disease.Long-term lack of effective control of high blood pressure could cause severe cardiovascular events such as coronary heart disease and heart failure.In addition to cardiovascular damage,high blood pressure could cause brain,kidney,eye and other target organ damage,these target organ damage was the main cause of death caused by hypertension.Comparison of normal group,hypertension group,hypertensive left ventricular hypertrophy group,hypertensive renal insufficiency group of low abundance proteome in the serum through the method of differential proteomics were analyzed by Tandem Mass Tag protein quantification and liquid chromatography-tandem mass spectrometry.The aim of this study was to investigate the role of proteins in the development of heart and kidney injury caused by hypertension,and to identify the proteins that may be useful by functional bio informatics network.In order to find the specific proteins that appeared in the process of heart and kidney injury,and to provide the new ideas for the prevention and treatment of hypertensive left ventricular hypertrophy and hypertensive renal insufficiency.Methods:Our study collected fifteen cases of normal people,hypertension patients,hypertensive left ventricular hypertrophy patients,hypertensive renal insufficiency patients with serum separately.Firstly,Proteins were analyzed and the differentially expressed proteins were screened out.Secondly,the bio informatics method was used to predict the differential protein.GO and KEGG were used for enrichment analysis.Finally,the specific signal pathways or protein markers associated with hypertensive left ventricular hypertrophy and hypertensive renal insufficiency were screened out.Results:When the normal group as a control,there were four specific proteins downregulated in the hypertensive patients with left ventricular hypertrophy.Specific pathways associated with cardiac hypertrophy were PI3K-AKT signaling pathway and P53 signaling pathway.There were fifteen specific proteins upregulated in the hypertensive patients with renal insufficiency.The specific pathway associated with renal function was renin-angiotensin system.When the hypertension group as a control,there were twelve specific proteins downregulated and two specific proteins upregulated in the hypertensive patients with left ventricular hypertrophy.No specific pathway associated with cardiac hypertrophy was found.There was one specific protein downregulated and eight specific proteins upregulated in the hypertensive patients with renal insufficiency.The specific pathway associated with renal function was the Rap1 signaling pathway.Conclusions:We have found specific proteins that may be associated with hypertensive heart and kidney damage,as well as the specific signaling pathways that may be present.These proteins may provide new ideas for the prevention and treatment of hypertensive left ventricular hypertrophy and hypertensive renal insufficiency.Object:Studies had shown that high density lipoprotein cholesterol levels could independently predict the poor prognosis and recurrence of stroke.However,the relationship between HDL-C levels and the cerebral thrombus was still unclear.The purpose of this study was to investigate the relationship between HDL-C levels and all-cause mortality in cerebral thrombosis patients.Method:A total of 768 cerebral thrombosis patients were recruited during 2000-2001 and prospectively followed up for a median of 4.5 years.There were 489(63.7%)male and the average age were(61.6±9.2).According to admission HDL-C concentrations,the patients were divided into 4 groups:Q1 group HDL-C concentrations?0.72mmol/L,n=182,Q2 group,HDL-C concentrations(0.72<HDL-C?0.86mmol/L),n=214,Q3 group,HDL-C concentrations(0.86<HDL-C?1.40mmol/L),n=196,Q4 group,HDL-C concentrations>1.00mmol/L,n= 176.The basic information was collected in all groups,the primary endpoint was all-cause mortality.Result:The all-cause mortality in the 768 patients with cerebral thrombosis was 20.4%(157/768).Q1 group was calculated to be set as a reference.The all-cause mortality in Q2(16.5%,36/142)and Q3(18.9%,37/196)group were lower than that in Q1(27.5%,50/182)group,the difference was statistically significant(P<0.05),but the all-cause mortality in Q4(19.3%,34/176)group was no significant difference to that in Q1(27.5%,50/182)group(P>0.05):With adjusted age,gender and other relevant factors,compared with Q1 group,Q2(HR=0.535,95%CI=0.343-0.835,P=0.006),Q3(HR=0.409,95%CI=0.255-0.65,P<0.001)and Q4(HR=0.497,95%CI=0.298-0.828,P=0.007)groups all had a protective role on all-cause mortality in cerebral thrombosis patient.Conclusions The levels of high density lipoprotein cholesterol were associated with all-cause mortality of cerebral thrombosis patients.Conclusion:The levels of high density lipoprotein cholesterol were associated with all-cause mortality of cerebral thrombosis patients.