Effects Of Hydrogen Molecules On Oxidative Stress In Liver Of High-fat Diet C57BL/6 Mice And Its Molecular Mechanism

Background:In recent years,the obesity-related diseases such as hypertension,type-2 diabetes,atherosclerosis(AS),fatty liver and cholelith have had the incidence gradually increasing,such diseases have been confirmed closely related to oxidative stress and excessive oxide free radicals are the common pathophysiological basis to induce the above-mentioned diseases,so to scavenge the free radicals can prevent or treat this kind of diseases associated with oxidative damages.Tests show that the hydrogen molecules,as a new type of antioxidants,can selectively remove the hydroxyl radicals and nitrate anions of strong toxicity in the oxide free radicals,so as to protect cells against oxidative stress damages,and moreover,have potent.In addition,the hydrogen molecules feature strong diffusivity and relative stability with no impact on the normal redox reaction signals in cells,thus able to prevent oxidative damages but not produce any side effects with interference in the cell functions.Currently,the hydrogen molecules are finding wider and wider applications in the medical field with the research involving in organ ischemia,arteriosclerosis,diabetes,organ inflammation,ischemia-reperfusion injury,senile dementia and a variety of other diseases,and the previous experiments all have proved the basis of the hydrogen molecules for oxidation resistance and also demonstrated that the hydrogen molecules have a variety of functions related to oxidation resistance such as anti-inflammatory performance,antiapoptosis and lipid metabolism regulation;furthermore,the preliminary experiments of our team also have proved that the hydrogen molecules can improve the lipid metabolic disorders in mice subject to a high-fat diet and have significantly reduced the levels of their blood lipid.Hydrogen can be widely applied in many fields by virtue of its advantages,such as easy preparation,low cost,quick action,easy operation and high permeability.So far,the experiments about hydrogen molecules have mostly been focusing on the oxidative stress for analyzing the therapeutic effect of hydrogen molecules,but the specific molecular mechanism is still worthy of further study.Moreover,further experiments currently shall still be needed to confirm whether any correlation exists among such functions of the hydrogen molecules as oxidation resistance,lipid reduction,anti-inflammatory performance and antiapoptosis and whether hydrogen absolutely has no any side effect on the human body.Objective:The current research has explored the impact of the high-fat diet and hydrogen molecules on the oxidative stress level of the C57BL#6 mouse livers and its mechanism as well as the relationship between the oxidative stress and the lipid and inflammation levels by determining the mouse body weight,blood lipids,liver tissue inflammation factors IL-6,8,10,TNF-aand oxidative stress indicators MDA and SOD as well as liver tissue HE staining.Method:Fifty C57BL/6 mice aged 6 weeks were randomly divided into two groups and were injected intraperitoneally with different doses of hydrogen saturated saline or normal saline for 8 weeks.The plasma levels of total cholesterol(TC),triglyceride(TG),high density lipoprotein(HDL-C),and low density lipoprotein(LDL-C)were measured by enzyme colorimetry.The contents of Malondialdehyde(MDA)and superoxide dismutase(SOD)were measured by enzyme-linked immunosorbent assay(ELISA)to evaluate oxidative stress level in the liver.At the same time,the levels of interleukin-8,10(IL-8,10)and tumor necrosis factor-alpha(TNF-a)were determined to reflect the inflammation level in the liver.HE staining was used to illustrate the effect of hydrogen molecules on the accumulation of fat in stem cells.Result:1.Compared with the normal saline group,the mice weight of hydrogen rich water group growth slowed down significantly,high fat diet group and normal diet group,hydrogen rich water group compared with control group the growth rate decreased by 44.02%and 58.43%,with statistically significant difference(P<0.01).2.In the diet group and the high fat diet group,the hydrogen enriched water group HDL-C increased by 48.73%and 20.13%respectively compared with the control group,and the difference was statistically significant(P<0.01).3.Normal diet group and high-fat diet group,hydrogen rich water group compared with the control group LDL-C were decreased by 10.66%and 17.03%;TC were decreased by 27.51%and 45.19%;TG were decreased by 12.44%and 15.31%,and the difference was statistically significant(P<0.05).4.Normal diet group and high-fat diet group,hydrogen rich water group compared with the control group MDA were decreased by 36.79%and 14.48%,with statistically significant difference(P<0.05);normal diet group SOD increased by 4.97%,the difference was not statistically significant,but showed a rising trend;high fat diet group increased 95.21%,statistically the significance of difference(P<0.01).5.Normal diet group and high-fat diet group,hydrogen rich water group compared with the control group IL-6 were decreased by 35.22%and 23.61%;IL-8 were decreased by 34.50%and 38.57%;IL-10 were decreased by 28.03%and 26.80%,TNF-a decreased by 41.97%and 20.24%,and the difference was statistically significant(P<0.01).6.Compared with the normal saline group,the hepatic lobule and stem cell structure is more complete in hydrogen rich water group,and the fat vacuole size reduction,high fat diet group and normal diet group,hydrogen rich water group compared with the control group of fat vacuoles area were decreased by 83.33%and 85.71%,with statistically significant difference(P<0.01).Conclusion:1.hydrogen molecule can regulate glucose and lipid metabolism.2.hydrogen molecules have anti-inflammatory effects.3.hydrogen molecules have the effect of anti oxidative stress.4.hydrogen molecules reduce the accumulation of fat in liver tissue.