The Effect Of Curcumin On The Proliferation, Invasion And Migration Of Human Lung Adenocarcinoma Cell Line A549 And The Expression Of HIF-1?

OBJECTIVE: The study was performed to investigate the anti-cancer mechanism of curcumin by estimating the inhibition ratio of cell proliferation,invasion,migration and the expression of HIF-1? on A549 cell.Methods:(1)The A549 cell was grown in RPMI1640 medium containing 10% fetal bovine serum(FBS)at 37oC in 5% CO2.(2)The A549 cell of logarithmic growth was subcultured for 3-4 times,which was used for next experiment.The experiment was divided into three experimental groups:24 hours-group,48 hours-group,72 hours-group.Each experimental group containing blank group and DMSO group was subdivided into five experimental groups by the curcumin concentration: 5umol/L-group;10umol/L-group;20umol/L-group;40umol/L-group;80umol/L.The absorbance values of each group were detected via MMT assay after the cell harvested at the end of the experiment.The inhibition ratio of A549 cell proliferation was calculated at last.(3)A549 cell of logarithmic growth was seeded in the Transwell chamber covered with matrixgel.The cell grown in the incubator at 37oC in 5% CO2 was treated with designed curcumin concentration for planed time.The cell stained was photographed with the microscope at the end of the experiment.The invasion of A549 cell was evaluated by the number of transmembrane cell.(4)The A549 cell of logarithmic growth was seeded in 6-well plate.The cell grown in the 6-well plate was scratched by sterile tip when the degree of cell fusion wasapproximately 80%-90%.The cell,rinsed 2 times with PBS,grown in the incubator was treated with designed curcumin concentration for planed time in the incubator at 37oC in 5% CO2.Pictures taken with the microscope were analyzed with Image-Pro Plus 6.0.The migration of A549 cell was evaluated by migration distance.(5)The A549 cell seeded into 96-well plates was treated with designed curcumin concentration for planed time in the incubator at 37oC in 5% CO2.The expression of HIF-1? of A549 cell was examined via enzyme-linked immuno sorbent assay(ELISA)at the end of the experiment.Results:(1)Compared with the DMSO group,curcumin suppress the proliferation of A549 cell,which was related to the concentration and treated time via MTT assay.There was a statistically significant difference(P<0.05).(2)Compared with the DMSO group,curcumin(except the 5umol/L-group)decreased the invasion of A549 cell,which was related to the concentration via transwell matrigel invasion assay.There was a statistically significant difference(P<0.05).(3)Compared with the DMSO group,curcumin(except the 5umol/L-group)decreased the migration of A549 cell,which was related to the concentration via wound-healing Assay.There was a statistically significant difference(P<0.05).(4)Compared with the DMSO group,curcumin decrease the expression of HIF-1? of A549 cell,which was related to the concentrations via enzyme-linked immuno sorbent assay.There was a statistically significant difference(P<0.05).Conclusion:(1)Curcumin suppress the proliferation of A549.(2)Curcumin decreased the invasion and migration of A549 cell.(3)Curcumin may inhibit A549 cell via regulation expression of HIF-1? protein.