Effects Of Electroacupuncture At Different Ganglion Acupoints On Gastric Function In OT Knockout Mice

Objective: To observe the effects of electro-acupuncture at different nerve segments acu-points for gastric emptying in OT gene knockout mice,explore the role of paraventricular nucleus OT in electro-acupuncture on different nerve segments acu-points to regulate gastric function.Methods: Twenty OT knockout mice(8-9 weeks old,male and female each half)and twenty wild-type mice were divided into control group,acupuncture “Zusanli”group,acupuncture “Neiguan” group and acupuncture Weishu group randomly,with 6 cases in each.The control group were treated without acupuncture,other three acupuncture groups were treated with unilateral electro-acupuncture at "Zusanli" “ Neiguan” "Weishu" point 15 min respectively,the other electrode was placed at the tip of the mouse when applying the electro-acupuncture.Subsequently,gastric emptying was measured by single photon emission computed tomography(SPECT)after gavaged milk loaded with technetium-99 m diethylenetriamine pentaacetic acid(99m Tc-DTPA),the gastric half-emptying time were calculated.Results:1.The wild-type mice,compared with the control group,electro-acupuncture at "Zusanli" and "Neiguan" can accelerate gastric emptying in wild-type mice(P < 0.05),and electro-acupuncture at "Weishu" could inhibit it(P < 0.05);2.The OT gene knockout mice,compared with the control group,electro-acupuncture at "Zusanli" and "Neiguan" had no significant effects for gastric emptying in OT deficient mice(P > 0.05),but the gastric emptying of electro-acupuncture at "Wei Shu" can still inhibit significantly(P < 0.01)3.Gastric emptying curve,gastric emptying in OT deficient mice was significantly faster than wild-type mice.Conclusion:1.The effects of Electro-acupuncture at different nerve segments acu-points for gastric emptying in wild-type mice have different degree.2.Knockout of OT gene can accelerate the gastric emptying in mices.And OT neurons of the paraventricular nucleus as the initiating device in the autonomic nervous pathways involved in electro-acupuncture at different nerve segments acu-points to regulate the gastric function,and inhibit the gastric emptying,and inhibit the gastric emptying.