Distribution Of Hepatitis-associated Virus In Neonatal Jaundice And Study Of Liver Damage

Objectives:1. To investigate the current status of jaundice among newborn in Zibo city.2. To find the main cause of neonatal jaundice viral infection, and to find the most suitable combined test set for neonatal jaundice virus infection.3. To determine the main virus that causes neonatal jaundice, and to analyse the correlation between neonatal jaundice and the virus resence in breast milk.4. To analyse the damages to liver function caused by infection of hepatitis associated virus in newborn.Materials and methods:We collected 1427 samples of neonatal blood and breast milk in the People's hospital of Zhangdian and the third people's hospital of Zibo City during February2013 and October 2014. Then all the blood sampes were detected for cytomegalovirus(HCMV), herpes simplex virus(HSV), hepatitis A virus(HAV), hepatitis B virus(HBV), hepatitis C virus(HCV), and epstein-barr virus(EBV) virus, ueing fluorescent quantitative polymerase chain reaction(PCR) and enzyme linked immunosorbent(ELISA). Then related viruses were detected in mother milk samples of neonatal jaundice mewborns retrospectively, and also the liver function indicators of virus positive specimens were detected using fully automatic biochemical analyzer test. All the datas were statistically compared with that of control groups.Result:1. 274 of all the 1427 blood samples were virus positive, 157 cases were HCMV positive(11.00%), 2 cases were HAV-Ig M positive(0.14%), 92 cases were HBV positive(6.45%), 3 cases were HCV-Ig M positive(0.21%), 12 cases were HSV-II-Ig M positive(0.84%), and 8 cases were EBV positive(0.56%). HCMV and HBV accounted for a higher proportion of viral infections which accounted for 11% and 6.45%respectively.2. 145 cases were HCMV positive in jaundice group, and 12 cases were HCMV positive in non-jaundice group, the difference was statistically significant(p < 0.01)between the two groups.3. 157 cases all the 1427 neonatal blood specimens were HCMV positive(11.0%), and1270(89.0%) cases were negative. 849 cases all the 1427 breast milk samples were HCMV positive(59.5%), and 578(40.5%) cases were negative. The difference was statistically significant(p < 0.01) between the two groups.4. Among aundice group, liver function indoctors such as TBIL, ALT, AST, and GGT of HCMV positive neonates were significantly higher than that of HCMV negative neonates(p < 0.01).Conclusion:The most common viruses that cause neonatal jaundice were HCMV and HBV in Zibo city, among which HCMV accounted for more than a half of all viruse infection,which needs more concerns. So we can first give detections for the two viruses,inorder to find the reasons economically and quickly. The HCMV positive rate(11%)of neonatal blood samples was lower than that of breast milk(59.5%). Therefore, it is meaningful to have an early dection of HCMV, which can reduce the infection caused by milk feeding. In the jaundice group, the liver function indicators of the HCMV positive patients such as TBIL, GGT, AST and ALT were significantly higher than that of HCMV negative patients that means that HCMV infection caused severe liver damage.