The Effect Of Chinese Herbal Medicine On The Proliferation And Migration Of Human Hepatoma Cells And The Expression Of HIF-1? And E-cad

Objective:To observe the effect of Rhizoma Paridis on proliferation?cycle and Migration turmor of hepatoma celldes an the expression of HIF-1a and E-cad.Methods:Cultured high transfer of human liver cancer MHCC97 H and Hep G2 cells in vitro experiments, Divided into three groups,respectively,Rhizoma Paridis group,Sorafenib group,blank control group, except the blank control group,the treatment group given corresponding drug intervention,using MTT assay,flow cytometry and effect of detection of different groups of drugs on cell proliferation and cell cycle in MHCC97 H cells;Using qualitative research durg scratch experiment of MHCC97 H and Hep G2 cellss under hypoxic training migration ability;Under the condition of hypoxia traning after drug intervention,the inverted microscope observation of cell morphology,real-time fluorescence quantitative PCR analysis the expression of HIF-1a and E-cad.Results :(1)MTT assay showed that different concentrations(0.2g/ml, 0.1g/ml,0.05g/ml,0.025g/ml) of Rhizoma Paridis in MHCC97 H cells showed the obvious inhibitory effect,different concentrations(50?mol/L,25?mol/L,12.5 ?mol/L, 6.25?mol/L) role of Sorafenib in MHCC97 H cells showed out inhibitory effect and in a concentration dependent manner,the maximum concentration of inhibition rate of Paris better than Sorafenib(P<0.05).(2) Flow cytometry 0.1g/ml Rhizoma Paridis to MHCC97 H cells in apoptosis ratio of 58.23%,better than 50mol/L Sorafenib tosylate(22.23%)and control group(4.74%)(P<0.05),and Rhizoma Paridis with apoptosis peak.(3) Scratch assay 0.1g/ml Rhizoma Paridis,50?mol/L of Sorafenib can inhibit invasion and migration ability of Hep G2 cells,and the blank contro l group(P<0.05).(4) Real- time fluorescence quantitative PCR detection of 0.1g/ml Rhizoma Paridis,50 ?mol/L Sorafenib compared with blank control(P<0.05)decreased as compared with the control group HIF1-a expression,including 50?mol/L sorafenib than 0.1g/ml Rhizoma Paridis(P<0.05); 0.1g/ml Rhizoma Paridis and 50?mol/L sorafenib,compared to the blank control group,E-cad expression significantly increased(P<0.05).Conclusion: Rhizoma Paridis can inhibit the proliferation of highly metastatic human hepatocellular carcinoma MHCC97 H cells induced apoptosis and inhibited the migration ability of human hepatoma Hep G2; Rhizoma Paridis can inhibit expression of HIF-1a and up regulation of E-cad expression;Rhizoma Paridis may through the link role in hepatocellular carcinoma epithelial mesenchymal transition and play a role of anti invasion and metastasis.