The Effect Of Stilbene Glycoside And Panax Notoginseng Saponins On The Inhibition Of PC12 Cells By A? 25-35

Objective:Polygonum multiflorum of tetrahydroxystilbene glycoside(TSG) and Panax notoginseng effective components of Panax notoginseng saponins(PNS) combined with anti- A beta 25-35 induced PC12 cells damage effective dosage, and mainly from the choline can system and oxidative stress pathways preliminary study of its mechanism, to provide a preliminary experimental basis for the clinical treatment of ad.Method:The first part, screening of beta amyloid peptide 25-35 optimum modeling concentration by using MTT assay. The PC12 cells as nerve cells,with a beta 25-35 induced PC12 cell injury model was established in AD,the cell survival rate as the index, the effective components of ad in PC12 cells damage effective inhibiting concentration. On the basis of evenly according to the U5(54) experimental design, 2*2 factorial design experiments determined two effective components of effective dosage.The second part, the PC12 cells by beta amyloid 25-35 induced establish injury model of Alzheimer's disease, were randomly divided into blank group, model group, positive control donepezil group(10umol / L), TSG(200 mmol / L) group, PNS group(50 mg / L), TSG-PNS compatibility group(TSG200 mmol / L PNS50 mg / L). Supernatant were measured byacetylcholinesterase(ACh E) and superoxide dismutase(SOD) activity,malondialdehyde(MDA) content and the calculation of each cell differentiation rate.Result:The first part: compared with the control group, 5 different concentrations of the model group cell survival rate were decreased(P<0.01), and with the A beta 25-35 concentration is higher, the lower the survival rate of cells(P<0.01).2 kinds of effective ingredients can improve the A ? 25-35 of PC12 cells induced by beta cell survival rate(P<0.05) and.U5(54) uniform design experiment of multiple regression analysis equation: 5 different concentrations and 2 effective components can survive rate of A beta 25-35 induced PC12 cells(P<0.05). The cell survival rate and TSG a linear relationship between PNS, TSG, PNS is a significant variable in the regression equation(P< 0.05). From the equation, TSG, PNS dose increased,the survival rate of the cells increased, while TSG under the experimental conditions, PNS, respectively with 200 mmol/L, 50 mg/L, the cell survival rate the highest factorial design. The experimental results showed:compared with the model group, TSG group, PNS group improved cell survival rate trends, but the difference was not statistically significant(P >0.05); the survival rate in TSG-PNS group increased significantly(P <0.05). The two factor factorial test results show that the main effect of TSG.PNS cells on the dependent variable The influence of the survival rate significantly(P = 0.046, P = 0.044), TSG and PNS interaction on the dependent variable cell survival rate had no significant effect(P = 0.053),showed that between the 2 main effects of TSG and PNS are independent.The second part:1. Compared with the blank group, the model group, the cell survival rate decreased significantly, and the difference is significant(P < 0.01);TSG group and PNS group and TSG-PNS group, the cell survival rate were higher than that of model group, the difference is statistically significant(P< 0.05), and the cell survival rate from high to low are as follows: TSG-PNS group and TSG group and donepezil group, PNS group(P < 0.01).Compared with the TSG-PNS compatibility group and TSG group, PNS group and donepezil group were lower than TSG-PNS compatibility group(P < 0.01, P< 0.05).2. Compared with the blank group, the ACh E activity in model group increased significantly(P < 0.01); compared with the model group, the activity of ache in each group were significantly lower(P < 0.05) and compatibility group and donepezil group than TSG group and PNS single group reduce more significantly(P < 0.01, P < 0.05), and the activity of ache from low to high followed by: TSG-PNS groups, donepezil group(TSG group, PNS group(P < 0.05). Compared with the TSG-PNS compatibility group and TSG group, PNS group and donepezil group were higher than TSG-PNS compatibility group(P < 0.01, P < 0.05).3. Compared with the blank group, model group, the activity of SOD significantly reduced(P < 0.01); compared with the model group. SOD activity in each group increased significantly(P < 0.05), the compatibility group and donepezil group were significantly higher(P < 0.01, P < 0.05),and compatibility group than TSG group and PNS single group increasedmore significantly(P < 0.01). SOD activity from low to high order: PNS group and TSG group and donepezil group, TSG-PNS group(P< 0.01).compared with the TSG-PNS compatibility group and TSG group, PNS group and donepezil group were lower than TSG-PNS compatibility group(P< 0.01).4. Compared with the blank group, model group, the contents of MDA increased significantly(P < 0.01); compared with the model group, MDA content in each group were significantly lower(P < 0.05) and compatibility group and donepezil group than TSG group and PNS single group reduce more significantly(P < 0.01, P < 0.05) content of.MDA from low to high followed by: TSG-PNS group and TSG group and donepezil group, PNS group(P < 0.01).compared with the TSG-PNS compatibility group and TSG group, PNS group and donepezil group were higher than TSG-PNS compatibility group(P < 0.01, P < 0.05).5. Compared with the blank group, model group, the cell differentiation rate was significantly lower and significant differences(P <0.05); compared with the model group, the cell differentiation rate in each group was significantly increased(P < 0.05), the compatibility group and donepezil group were significantly higher(P < 0.01, P< 0.05), and compatibility group than TSG group and PNS single group increased moresignificantly(P < 0.01). Cell differentiation rate from low to high as follows: PNS group and TSG group and donepezil, compatibility group of TSG-PNS group(P < 0.01) and TSG-PNS and TSG group, PNS group and donepezil group were lower than TSG-PNS compatibility group(P< 0.01).Conclusion:Stilbene glucoside TSG 200 mmol / L and Panax notoginseng saponins PNS 50 mg / L of the prescription of beta amyloid peptide 25-35 injury in PC12 cells injury has obvious synergistic role.TSG and PNS treatment of ad mechanism may be through improving choline can damage, restrain the oxidative stress injury and protect the nerve cell synaptic plasticity and to achieve.