Expression Of Protein Arginine Methyltransferase 1 In Hirschsprung's Disease

Objective:Protein arginine methyltransferase1(PRMT1) catalyzes a large proportion of arginine methylation in cells.PRMT1plays important roles in the development and differentiation of neurons. Hirschsprung disease (HD) is characterized by the absence of ganglion cells in distal gut. In this study,we will detect the PRMT1expression in the colon segments from HD patients and control group. We will study the pathogenesis of HD.Methods:Tissues samples were obtained from forty patients (twenty HD patients and twenty non-HD patients) from September2010to October2011. Samples from HD patients were collected from aganglionated segments. Samples from non-HD patients were collected from normal segments. The forty patients underwent surgical procedure at the Liaocheng People’s Hospital and the Second People’s Hospital of Liaocheng City. Tissue samples were collected immediately after operation and were stored at-80℃until use. Western Blot analysed the PRMT1protein levels in colon segments Immunohistochemistry was used to detect PRMT1expression in different layers of colon tissue cell from HD patients and control group.Result:Reduced PRMT1protein was found in the segments of HD patients (p<0.05). Strong PRMTl expression was detected at all crypt cells. Intensive PRMT1expression was detected in both the submucosal and the myenteric plexuses from the control group. The colon segments of HD patients were not markedly labeled with PRMT1(p<0.05). This is the obvious characteristic in HD patients。Conclusions:PRMT1expression should be used to investigate the pathogenesis and as a useful marker for the diagnosis of HD.