The Effect Of Dietary Arginine Addition On The Regulation Of Innate Immunity And Antioxidant Capacity In Broilers

In the present study, The experiment was conducted to investigate the effect of dietary arginine(Arg) supplementation on innate immunity and antioxidantability of broiler chickens. which was research Arginine regulates immune function in broilers.It provide data to environment control and premiun chicken meat production. While it has important theoretical guidance for arginine in broiler production in reasonable applications.This study was included two partsExperiment 1:The experiment was conducted to investigate the effect of dietary arginine(Arg) supplementation on innate immunity.It was designed as a single-factorial arrangement(n 8 cages/treatment,6 birds/cage) with four dietary Argconcentrations (10g/kg,15 g/kg,20 g/kg or 25 g/kg).Respectively in the first,14 and 21 days did the statistics analysis. On day 21 birds were killed to obtain spleen bursal cecal tonsil for gene expression and collection serum for ELISA.In theexperiment, the results showed increasing dietary Arg supplementation linearly and quadratically increased (P<0.05) body-weight gain (BWG) and decreased (P<0.05) feed conversion ratio (FCR), and quadratically increased (P<0.05) feed intake (FI); linearly reduced (P<0.05).The result showed that dietary supplementation of Arg linearly increased (P<0.001) the expression of MIP-1? and IFN-y mRNA in the cecal tonsil. Dietary supplementation of Arg quadratically decreased (P<0.05) the expression of IL-1? and IL-6 mRNA in the spleen. linearlyreduced (P<0.05) serum IgG andIgM concentration, mucosal IgM concentration. NC and LA/NC were linearly decreased (P<0.05) with increasing Arg supplementation. LC trended to be linearly increased (P<0.10) by increasing Arg supplementation. With the increasing Arg supplementation, LC trended to be linearly increased (P<0.10).Experiment 2:The experiment was conducted to investigate the effect of dietary arginine(Arg) supplementation on antioxidantability of broiler chickens.It was designed as a single-factorial arrangement(n 8 cages/treatment,6 birds/cage) with four dietary Argconcentrations (10g/kg,15 g/kg,20 g/kg or 25 g/kg). On day 21 birds were killed to obtain liver for determination of antioxidant index.Dietary Arg supplementation linearly (P<0.05) increased the GSH-Px (glutathione peroxidase),CAT(catalase),LZM(lysozyme) activity in the liver. In the study, the results indicate that high level of Arg supplementation (>20.0g/kg) suppressed innate immunity of broiler chickens. Furthermore, increasing dietary Arg concentration enhanced antioxidant activity of broiler chickens.Above all, the present study demonstrated that high level of Arg supplementation (>20.0g/kg) may potentially suppress the innate immunity of broiler chickens,and dietary Arg supplementation enhanced the antioxidant activity ofbroiler chickens.