Digital Droplet-flow Nucleic Acid Detector Control System Research

Malignancy and AIDS are two major hazards to human health.Every year,roughly 10 millions die of malignancy,and another 2.3 millions are infected with HIV.The pathogenesis,prevention and treatment of malignancy and AIDS have become much-discussed research topics among various countries.Many domestic researchers also dedicate their research into the pathogenesis and treatment of malignancy and AIDS.However,the limited research methods set an apparent bottleneck for relevant research advances.Therefore,the research arena requires a new nucleic acid detector equipped with high sensitivity and absolute quantification.Supported by the horizontal project "Digital Droplet-Flow Nucleic Acid Detector",this dissertation concluded the development of control system of Digital Droplet-Flow Nucleic Acid Detector,and conducted a whole-machine joint debugging for the designed control system.The main content of this dissertation can be described as follows:(1)Explain features(e.g.principles,detecting methods,etc.)of Digital Droplet-Flow Nucleic Acid Detector.(2)Determine basic functions and overall design of Droplet Digital-Flow Nucleic Acid Detector based on the design of the whole machine.(3)Complete the design of hardware circuit for the control system of Digital Droplet-Flow Nucleic Acid Detector based on STM32.The Master Controller Unit comprises microprocessor module,power module,communications module,etc.(4)Code on MDK the software program for the control system of Digital Droplet-Flow Nucleic Acid Detector.The main system modules consist of system initialization,syringe pump control,rotary valve control,stepping motor control,etc.(5)Finish tests for each module,including tests for circuit in the control system,experiments for Digital Droplet-Flow Nucleic Acid Detector and others.A whole-machine joint debugging for the designed control system and other working modules is conducted in this dissertation.The results indicate that the designed control system is able to coordinate different modules in the Digital Droplet-Flow Nucleic Acid Detector to achieve expected functions,and to guarantee the stability of the Digital Droplet-Flow Nucleic Acid Detector.