The Construction Of Copper-based Nanomaterials And Their Applications In Photoacoustic Imaging

As an emerging imaging technology in medical diagnostics,photoacoustic imaging?PAI?technology has received more and more attention and research.Photoacoustic imaging has high resolution and high contrast.Based on this,it collects and analyzes the molecular information of the tissue.And through reconstruction,resolution,and other steps,highly specific images of organs and tissues can be obtained.In combination with molecular imaging probe technology to enhance imaging contrast,PAI technology has great advantages in imaging at living level.Through the addition of contrast agents,PAI technology can produce imaging effects suitable for medical diagnostics,enabling non-invasive,multi-faceted,multi-contrast visualization imaging.Of the many contrast agents currently studied,contrast agents based on copper sulfide nanomaterials have particular advantages.Copper sulfide nanomaterials are readily available without the need for complex synthetic processes,which greatly reduces the cost of treatment;particle size has a good regulatory effect on the pharmacokinetics of the material in vivo and tumor accumulation and other issues.Through the adjustment of particle size,it was found that the copper sulfide nanomaterial still has a strong near-infrared absorption at a size of 2 nm.This paper includes the following two parts:The first part:We first prepared and characterized the CuS@BSA nanomaterials,and carried out photo-thermal experiments at the solution level,photoacoustic imaging experiments and so on.It was found that CuS@BSA has strong photothermal performance and strong photoacoustic imaging effect,which is very favorable for photoacoustic mediated photothermal therapy in vivo.In view of the active phagocytic ability of macrophages to foreign bodies and good inflammatory tropism,we prepared a macrophage-mediated CuS@BSA composite nanomaterial.The macrophage-mediated CuS@BSA composite nanomaterials were successfully obtained by LSCM verification.Next,we established a mouse breast cancer tumor model?4T1?,and then explored the photoacoustic imaging and pharmacokinetic effects of CuS@BSA at the living level.The good photothermal performance and photoacoustic imaging effect of the composite material make it have a good application prospect in photoacoustic-mediated tumor targeted photothermal therapy.The second part:We prepared and characterized Cu₂O nanomaterials with small particle size,and carried out competitive particle experiments,near infrared absorption experiments,photoacoustic imaging experiments,cytotoxicity experiments,and hemolysis experiments at the solution level.The material was found to detect hydrogen sulfide gas in vitro and produce a specific photoacoustic signal response,and its low cytotoxicity allow the tests in vivo.At the mechanism level,the reaction of Cu2O nanomaterials with H2S was investigated.On this basis,we established a model of mouse colon cancer tumors and successfully achieved specific detection of endogenous hydrogen sulfide in mouse tumors.