| With the developing of technology,interface/surface functionalization plays an important role in both natural and artificial materials.Therefore,the development of surface-functional strategies is significant for various fields.Amyloid adhesion is a newly-developed method which inspired by biological adhesion of barnacles and microbial.However,the mechanism of amyloid adhesion is still unclear.In this study,we focused on the mechanism of amyloid adhesion of lysozyme phase transition system on different substrates.On the other hands,the protein based coating exhibited excellent biocompatibility and broad-spectrum antibacterial,which offered a new strategy of antibacterial for various materials.The research outline is as follows:(Ⅰ)The adhesive mechanism of lysozyme phase transition systemPioneering works of our group indicated the lysozyme could undergo a phase transitional process with TCEP(tris(2-carboxyethyl)phosphine)treated,which produced lysozyme based nanofilm on various surfaces with inner amyloid structure.In this experiment,the adhesive properties were characterized by coating adhesion automatic scratch tester.The results showed that the adhesive behaviors based on the aspects:the properties of phase-transited lysozyme,thickness of the nanofilm and the functional group of substrate surfaces.On the other hands,we investigated the adhesive interactions between the functional groups(-NH2,-COOH,-OH,-SH,-(CH)n-CH3,aromatic)of phase-transited lysozyme and the different type of substrates.Besides,the coordination,electrostatic interactions,hydrogen bond,hydrophobic force of the amyloid structure also provided strong interactions for the adhesions.In brief,the multiple interactions between the film and substrate provides a new strategy for surface functionalization.(Ⅱ)An environmentally benign antimicrobial coating based on PTL nanofilm adhesionWe report a class of antimicrobial PTL nanofilms,with broad-spectrum antimicrobial action toward Gram-positive/negative bacteria and fungi as well as antifouling and antibiofilm activity without the loading of any other environmental hazards.without the loading of any other environmental hazards.Such performance was attributed to an intrinsical triple-combination of positive charged and hydrophobic residues as well as surface hydration effect in the protein nanofilm.The amyloid-like structure in the PTL material boosts the reliable bonding with virtually arbitrary materials/devices in minutes.In vitro cytotoxicity assays indicate that the proteinaceous coating favors good hemocompatibility and cytocompatibility toward mammalian cells.An in vivo antimicrobial test on invasive implants indicates that this proteinaceous coating on an implanted catheter presents an excellent infection inhibition effect in a rat model.The antimicrobial results shown herein illustrate how protein selfassembly could be manipulated to provide a new class of antimicrobial nanomaterial with increased activity.PTL nanofilm has opened a new way of biomolecular assembly engineering towards green sustainable antimicrobial biomaterials. |
