Study On Treatment Of Sulfate Reducing Bacteria In Heavy Metal Mine Wastewater

With the rapid development of the economy,the demand for metal resources is increasing.In the process of mineral resources exploitation,the natural gas of the sulfide ore meets the air,and enters the environment with the water inflow and the leaching water.The mine wastewater containing heavy metal and sulfate is formed.As mines are mostly located in mountainous areas and upstream of drinking water sources,they pose a major threat to the safety of drinking water supplies.In this study,sulfate reducing bacteria biochemical treatment of mine wastewater,the sulfur component metabolism and sulfur balance system of the reduction process for wastewater of heavy metal ions in formation of sulfide precipitation metabolic regulation guidance;study the regularity and mechanism of interaction involving sulfur component and pH,redox potential and other monitoring indicators,provide the real-time monitoring and control for anaerobic biochemical treatment;through the microbial electron donor,toxicity inhibition products,trace component research,implementation of sulfate reduction bacteria optimization of mine wastewater treatment,provide technical support for the mine wastewater finally realize the green,efficient processing.(1)The results showed that mixed strains used in the study were identified as Desulfovibrio,analysis system involving sulfur components:water sulfate,sulfide,sulfur water microbial components,Wade sulfur component,set up a set of effective separation of various measurement methods involving analysis of sulfur components.The biochemical reaction of sulfate reducing bacteria to sulfate reduction and metabolism,the intermediate product to the final product sulfur ion is the rate limiting step,and there is no continuous enrichment of sulfur components in the microbial cells.At the end of the reaction,the major sulfur forms in the system accounted for 5.8%of the total sulfur,15.7%of the residual SO42-,76.9%of the S2-,2.2%of the microbial sulfur,and the middle of the sulfur containing component.(2)The results show that:in the SRB sulfate reduction system,the concentration of SO42-does not affect the pH value and ORP value;sulfate reducing bacteria of sulfate metabolism in pH=7.2,near ORP=-270mV are turning,monitoring indicators shows that the accumulation of metabolic intermediates began to appear.After pH=7.5 and ORP=-160mV,the speed limit is relieved.For SRB systems with different Cu2+ concentrations,the lower the pH value and the higher the ORP value,the more detrimental to the growth of SRB with the increase of Cu2+ concentration.(3)Studies on microbial metabolism,carbon source,trace element stimulation and toxicity inhibition were carried out.The results showed that in the study of carbon source types,the order of SRB removal was:sodium lactate>glucose>white sugar>starch.The addition of Fe0 contributes to the sulfate reduction process.Adding vitamin C helps SRB to restore sulfate.By intermittent addition of Fe2+ and Cu2+,the toxic inhibition of sulfur ion can be effectively relieved.