| Analytical an indispensable technique for human to know the composition and structure of substance. In modern analytical technology and methods, chemiluminescence, due to its high sensitivity, wide linear range, simple and cheap equipment, easy access to miniaturization and automation, has become a reliable and effective analysis method in environmental science, food safety determination, biomedicine etc. Therefore, chemiluminescence sensors that use sensitive chemiluminescence reactions as reactions has drawn great attention from the analysis operators, and has become one of the research hotspots on sensor. However, the characteristics of chemiluminescence sensors reported so far are still not perfect. Therefore, it is necessary to research and develop new design ideas, new chemiluminescence systems and new reagents immobilized methods to make them obtain such characteristics as long-term preservation, long usable life and good selectivity etc., and finally make chemiluminescence sensors truly go out of the laboratory and realize commercialization. Chemiluminescence sensor is based on chemiluminescent reaction and most of the chemiluminescent reactions are oxidation-reduction reaction, so oxidation agent is an important part of the chemiluminescence reaction. Based on the above knowledge, this paper focuses on the oxidant immilized chemiluminescence sensors, and structures a kind of chemiluminescence sensors with the characteristics of long-term preservation, long usable life, and within easy access to commercialization. In order to verify their reliability and practicability, they were applied to the reported chemiluminescence's system. The papers covers the following aspects:The first chapter is a review of the thesis, elaborating the research history and principle of chemiluminescence and a general description of the research history, classification, immobilizing methods and carriers of chemiluminescence sensors. Finally, it states the research background, research contents and this paper's innovations.In the second chapter, it establishes a new Mn3+ release chemiluminescence sensor. The sensor used water-soluble phosphate glass as the carrier of Mn3+. Mn3+ was prepared and immobilized by means of high temperature heating. In order to verify the sensor's reliability and practicality, it was applied to detecting analgin. The experiment shows that the response to analgin concentration was linear in the range of 3.0×10-8?1.1×10-5 mol·L-1 with a detection limit of 3.0×10-10 mol·L-1, the relative standard deviation RSD is 1.6% in 11 repeated. The advantages of the sensor are its simple preparation method, low cost, can be stably stored for a long time. What's more, through control the dissolution solution concentration and volume to control oxidant concentration, and then control the life of the Mn3+ release chemiluminescence sensor.The third chapter structures a NaBiO3 release chemiluminescence sensor which uses cement as the carrier. The sensor immobilized NaBiO3 with strong oxidation in cement using the principle of hardened cement. In acidic medium, NaBiO3 can oxidize inorganic and organic. Based on this phenomenon, this experiment studies different organics, such as isoniazid, analgin, perphenazine, amoxicillin and cefradine chemiluminescence behavior in this system. The linear ranges achieved were 5.0×10-7?1.1×10-5 mol·L-1 isoniazid, 5.0×10-8?1.1×10-5 mol·L-1 analgin,5.0×10-7?9.0×10-6 mol·L-1 of perphenazine, 9.0×10-7-1.0×10-5 mol·L-1 of amoxicillin and 9.0×10-7?1.1×10-5 mol·L-1 cefradine. The detection limits were 2.2×10-8 mol·L- isoniazid,3.1×10-10 mol·L-1 analgin,1.6×10-8 mol·L-1 perphenazine,5.6×10-8 mol·L-1 amoxicillin and 2.3×10-8 mol·L-1 cefradine, the relative standard deviation RSD in 11 repeated are less than 5%. The experiment shows that, the sensor has high reliability and stability. The sensor, with long usable life and long-term preservation, can be simply prepared and is easy to replace. It has a broad application prospect in the on-line analysis of the inorganic and organic compounds which have reducibility.The fourth chapter summarizes the main content of this paper, pointing out the advantages and disadvantages of this research, and puts forward the outlook of the future work. |
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