Based On Bone Age Analysis, The Age Structure Of The Population Of Qinling Rain Frogs And Their Populations Were Compared

Hyla tsinlingensis belongs to the phylum Chordata, class Amphibia, order Anura, family Hylidae, and genus Hyla. Hyla tsinlingensis is endemic to the Qinling Mountains and Dabieshan Mountains of China. Their body lengths are 40mm or so, and there are adhesive pads and horseshoe-shaped groove edge at the end of fingers and toes, which gives them the ability to adsorb on things like leaves and blades of grass. They inhabit at altitudes ranged from 830m to 1770m.In order to supplement the life history studies of Hyla tsinlingensis, this study got the composition and distribution of age structure, age of sexual maturity, generation time and maximum service life of the Hyla tsinlingensis, based on the basic characteristics, the growth curve and age. At the same tine this paper compared and analyzed the difference and causes between populations, so as to understand Hyla tsinlingensis current state of life in the Qinling mountains and Dabieshan mountains. This study will help us understand the life history strategy of the species and the relationship with environment, and then provide a the relationship with environment, and provide a theoretical basis for the regional protection of the species.A total of 236 specimens were collected from 15 sampling sites that locate in the Qinling Mountains and Dabieshan Mountains. The skeletochronological method was used to determine the ages. The independent sample t-test, one-way ANOVA and Pearson’s correlation analysis were used to analyze the relationship of body length, body weight and the age between males and females. Individuals from a sampling site are treated as a locale population, and all local populations were divided into two large populations including the Qinling population and the Dabieshan population. The linear correlation of individuals’ages and body lengths between male and female was analyzed for different altitudes. The population age structure, the age at sexual maturity and the generation time were also analyzed. The growth curve was obtained by regressive analysis using the von Bertalanffy’s equation, St= Sm-(Sm-S0) eK (t-t0).1. The results also showed that with the elevation increase, it is not consistent with Bergmann’s law.2. According to the results of skeletochronological studies, the age of sexual maturity of Hyla tsinlingensis is 2 years old, regardless of sexes or regions. Combining toe bones paraffin sections and HE staining, the study identified the age of 210 males and 10 females successfully, and divided them into three age groups namely 1,2,3 age group. The results showed one age of Hyla tsinlingensis account for a higher proportion of male and female sex.3. The study also found that, the toe sections showed Hyla tsinlingensis individuals between the second LAG and the third LAG have a narrowed distance significantly, so adult were matured and started to enter the reproductive stage in the second year after metamorphosis, so we judged its mature age is 2 after perverted. The generation time is 2 years.4. By the von Bertalanffy equation St= Sm-(Sm-S0) e-K(t-t0), we get the growth curve and regression equation y=39.55-(39.55-14.97) e-3.149x, r2=0.014 (female) and y=32.01-(32.01-14.97) e-2.492x, r2= 0.472 (male). The female growth rate K= 3.149, male growth rate K= 2.492. Obviously, females grow faster than males. Hyla tsinlingensis maximum lifespan is about 3 years old, the age of frogs belonging shorter. When Hyla tsinlingensis is 2 years old that is mature age after sexual perversion, male and female individual growth rates will slow down. The body length could not be used alone to determine the age since the body length is about the same in the three age groups.5. According to analysis the male morphology of 15 populations, use found significant differences exist between populations of Hyla tsinlingensis; simultaneously, comparing Qinling mountains and Dabieshan mountains populations found that the significant differences are also exist between two regions. The reason for this is clue to different geographical conditions, lining conditions and the extent of human interference, and this difference is the result of combined effects of conditions.