Analysis Of Antibacterial And Antitumor Activity Of Endophytic Bacteriocin

Prodigiosins, is a typical alkaloid compound produced by some Actinomycetes, bacteria. Prodigiosin is of great interest due to its antibacterial, antifungal, antimalarial properties, algicidal effect immunosuppressive activity, inhibit cancer cell metastasis, etc. The experiment was about the strain HUB-?-107 isolated from a natural anti-termite trees (Chamaecyparis lawsomcma) that fermentation metabolites separated and purified prodigiosin, investigate physicochemical properties,bacteriostatic activity, antineoplastic activity of prodigiosin.1. Obtained strain HUB-?-107 prodigiosin, its molecular formula for C20H25N3O, and molecular weight of 323, It easily dissolved in organic solvents such as alcohols, DMSO. It is relatively stable under acidic conditions (pH=3). And it has good resistance to reduction, oxidation resistance and thermal stability.2. Strain HUB-I-107 prodigiosin antibacterial test results show that, Prodigiosin has a on certain inhibitory effect on Enterobacter aerogenes, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Enterobacter cloacae, especially Staphylococcus aureu.3. The tumor cells test results showed that the, strain HUB-?-107 prodigiosin has inhibitory effect on the A549, HeLa, HepG2, LNCaP, MCF-7, IC50 of 40?g/mL, and it's showed a time-dose-dependent manner.4. FCM detect the influence of cell cycle under strain HUB-?-107 prodigiosin, it turned out that the strain HUB-?-107 prodigiosin inhibited further cell division and proliferation through inhibiting replication of DNA in S phase.5. Detect the expression of Caspase-3 by western blot, test results show that, activation of Caspase-3 induced apoptosis of A549.6. Antitumor in vivo test results show that with increasing the time and the concentration of strain HUB-?-107 prodigiosin, tumor volume was significantly reduced, and has small differences between normal cells and tumor cells, it indicated that the strain HUB-?-107 prodigiosin restrained vivo tumor inhibition in a dose-time-dependent.