Preliminary Analysis Of The Morphological And Projection Targets Of Pyramidal Neurons Of Primary Motor Cortex In Mouse Brain

Understanding the structure and function of the brain is the most important in today's neuroscience.As the basic unit of the brain,neurons connect to each other,forming a highly complex neural network.Understanding the structure of different types of neurons and the relationship of network connectivity is critical to understanding the working principle of the brain.And the primary motor cortex as an important component of the cerebral cortex.Therefore,analysis of the pyramidal neurons of the primary motor cortex will be an important and indispensable part of constructing the neuronal morphological database and the whole brain network connection map.The thesis has selected the pyramidal neurons in primary motor cortex as the research object.Based on the Micro-Optical Sectioning Tomography system and GFP staining technique,we get the whole brain dual channel data set with high resolution,a standardized process for long-range neurons from the localization of brain region,the localization of neuronal cells,and the reconstruction of its complete morphology to morphological and projection patterns were established.Firstly,a method based on two-channel image registration and image fusion was used to get the location of brain and cell bodies.And based on the localization results,we reconstructe the three-dimensional digital morphology of 44 pyramidal neurons in primary motor cortex.Then,the obtained pyramidal neurons are classified based on a method of neuron dendritic enrichment.And the results were validated by calculating the thermogram and length density curves of neuron dendrites.Finally,the axonal projection targets of long-range neurons were mapped based on the reconstructed data and the PI channel image.And realizing the location of long-distance neuron projection at brain region level in primary motor cortex.The study of the pyramidal neurons in primary motor cortex that further improved the neuronal morphological database and the network connection map at single neuron level.And study pipelin in this paper can be expanded and applied to other brain area even to the whole brain,which lays the foundation for studying the structure of the brain.