Design And Experimental Study Of Cell Culture Chip With Surface Microstructure

There are many kinds of highly ordered microstructures in living organisms,and the biological functions unique to cells,tissues and organs are closely related to the microstructure.Therefore,the simulation of human microenvironment in vitro culture of cells is an important basis for drug screening and in vitro pathology.At present,there are mainly two-dimensional culture and three-dimensional culture in vitro.Two-dimensional culture is simple,but can not truly reflect the biological characteristics and functions of cells in the body.Three-dimensional culture of cell technology refers to the use of extracellular matrix formation of cell growth scaffolds,so that the cells in the three-dimensional structure of migration,growth and differentiation to produce a certain three-dimensional organizational structure,can better simulate the cells in vivo microenvironment.Based on this,a novel method of manufacturing surface micro-structure based on biofunctional materials was developed based on the project of "3D Printing Technology of Microstructure of Biofunctional Materials",an open fund project of State Key Laboratory(GZKF-201513).The design of the cell culture chip based on the surface microstructure and the related experimental research were carried out to realize the cell dynamic culture while arranging the cells.The research work of the thesis will provide a new design idea for the structure design of bionic in vitro cell culture chip.It will be instructive for the fabrication and experimental study of biomimetic surface microstructure based on bio functional material.It can also be used for personalized drug screening and in vitro Pathology and other tissue engineering and medical research to provide technical support.In Chapter 1,The background and significance of the research in this paper were expounded.The research status and development trend of the related technologies such as cell culture chip structure design,surface micro-structure manufacturing method and other related technologies were reviewed.The research contents and the frame structure of this paper were proposed.In Chapter 2,The design scheme of new cell culture chip based on the surface microstructure was proposed,and the overall structure of the cell chip was designed.Based on this,the working principle of the cell culture chip was analyzed,the appropriate flow rate was selected,and the hydrodynamic analysis of the cell culture chip was performed.In Chapter 3,For the design of the surface microstructure,a method based on the combination of surface acoustic wave(SAW)and UV curing microstructures was proposed.By analyzing its forming principle and using Matlab to simulate the acoustic pressure field distribution under different working conditions of the transducer.Then the structure design of the SAW transducer was carried out and the fabrication of the interdigital transducer device and the forming system were completed.In Chapter 4,The bio-functional materials were prepared and the surface micro-structure was formed by using the formed manufacturing system.In addition,the influence of process parameters on the surface micro-structure forming was studied.On the basis of this,the stability of the surface micro-structure of the bio-functional material in liquid environment was tested.In Chapter 5,The prototype of the cell culture chip was fabricated by the process of layering and reassembling the package.The effects of different culture methods on the proliferation and survival rate of L929 mouse fibroblasts were analyzed by cell culture experiments.The effect of different microstructure spacing and morphology on the arrangement of L929 cells was also studied.In Chapter 6,the main research work of the dissertation was summarized,and the future research work were proposed.