Research On Detection Techniques For Porcine Circovirus Type 2 Based On Excessively Tilted Fiber Grating

Biochemical immune sensor has been a very active research in the field of sensor technology.Especially in recent years,with the development of various new principle and the advancement of new technology,biological immune sensor is moving toward miniaturization,integration,intelligence.At present,the traditional biological immune detection method is due to the longest time,the high cost of testing,bulky instrument,expensiveness and needing professional characteristics,not conducive to miniaturization,integration,real-time online monitoring.However,fiber Bragg grating sensor not only has the advantages of small volume,anti-interference,corrosion resistance,but also has the characteristics of a "pass" and "feeling" transmission at the same time.And that shows a broad application prospect,such as the disease diagnosis,biological information research and the analysis of genetic testing.Therefore,this paper,based on excessively tilted Fiber Grating as the platform for biological immune detection,using chemical methods to modify staphylococcus aureus A protein on the surface of excessively tilted Fiber Grating,then using Porcine circovirus virus antibody immune markers to construct a layer of bio-film which can specifically recognize the corresponding antigen used for detection analysis.By spectral data analysis,immunology angle,image characterization and so on to analyze the biochemical immune sensor's performance.In this paper,the main research contents include the following:(1)Designing and producing the excessively tilted Fiber Grating,and going on experiments to detect refractive index sensitivity of the sensor,establishing the relationship between the concentration and refractive index.(2)Based on the excessively tilted Fiber Grating,to build biological immune platform and to study the surface's modification technique;under the auxiliary of scanning electron mirror,fluorescence microscope,spectrum demodulation devices to study the immune sensor.Knowing that the number of the site on the sensor's surface was to restrict the detecting range and precision.(3)Constructing biological immune detection system for studying the biochemical immune sensors of low detection limit,maximum saturation,detection range.The results respectively reach to ~10 TCID50/m L,~ 4800 TCID50/m L,10-4800TCID50 /m L.In addition,this designed biochemical immune sensor can disintegrate,which shows a good repeatability.(4)Combing with the principle and experiment data of biochemical immune sensors,the Mie equation model is established.Meanwhile,analysing the mechanism and calibrating the meaning of each parameter provide a powerful method for the biological immune sensors.This paper researches biochemical immune sensor based on the excessively tilted Fiber Grating,which is a extremely important complement in the field of biological immune detection.It has a very low cost,simple operation,convenience to carry,more suitability for micro-structure,small size,ultra trace detection,which provides a new method in biology,medicine,food,environment and other aspects.