Establishment And Application Of Cell Adhesion/Sensing Interface

Since the 80's of the 20th century, cell-based biosensor has become a frontier and hot spot in the field of cell biology and biological monitoring technology due to its powerful vitality. However, how to make sensing interface to be specific for living cells adhesion has always been the key factor that affects the sensitivity and precision of the cell-based biosensor. In this work, several kinds of bioactive cell adhesion molecules were used for modifing the sensing interface favorable for specific adhesion of animal cells, which would promote the future development of cell-based biosensors. The following is a summary of the main results.(1) A compact and stable interface for specific adhesion of living cells was prepared through the self-assembly technology. The RGDC which can improve cell adhesion and PEG which prevents non-specific adhesion were decorated on the Au electrode. The adhesion efficiency of rat cardiac myocytes (H9C2) onto the interface was studied by adjusting the ratio of RGDC and PEG. It is found that when the RGDC and PEG molar concentration ratio is 1:1, the interface demonstrated its best performance for specific adhesion of H9C2 cells. In addition, we also investigated the electrochemical behavior of H9C2/RGDC/PEG/Au sensing interface in the aqueous solution of chromium (VI). The constructed cell specific adhesion interface and electrochemical analysis technology is expected to be very useful in studies on the toxic effects of heavy metals on living cells.(2) A compact and stable FN/MPA/Au electrode sensing interface of specific adhesion to HUVEC cells was prepared by self-assembly technique coupled by chemical crosslinking. The conditions for the modification of MPA and the subsequent FN coupling were optimized. The results showed that the interface built under the conditions of 20 mmol-L"'MPA with 12 h modification time, 20 ?g-mL-1 FN is the best for HUVEC cell adhesion.(3) Electrochemical polymerization followed by chemical coupling was used to prepare FN/4-HPA/ITO sensing interface of specific adhesion to living cells with good transparence. And optical microscopic observation indicated that the modified FN could promote cell adhesion and spreading.In summary, the FN/4-HPA/ITO interface can promote adhesion of animal cells to the sensing electrode, amplify the measured signal of living cells while maintaining the cells' activity, moreover it allows the electrode to combine with an optical microscope to confirm the measured signal, therefore, it is expected to be applied widely in the area of cell-based sensors.