Preparation Of Thin Film Luminescence Sensor And Its Application In Detection Of Dyes

With the continuous development of human society, dye wastewater pollution is becoming more and more serious. Various types of enterprises to increase the discharge of industrial waste water and some organic dyes can cause damage to our health by the food chain directly or indirectly. Therefore, it is essential to study a kind of efficient, sensitive and accurate sensor to detect organic dyes. Thin film luminescence sensor is a new type of fluorescent sensor. Compared to other fluorescent sensors, it has the advantages of quick, high sensitivity, strong fluorescence and high precision. What is more, it can be reused. In this paper, we mainly study the thin film luminescence sensor, which has been successfully prepared and detected Rhodamine B and Fluorescein Sodium by using qualitative and quantitative methods. And the following conclusions are drawn:(1) The fluorescence control of upconversion nanoparticles(UCNPs) was achieved. Uniform upconversion nanoparticles(UCNPs) with uniform size and intense visible fluorescence were successfully synthesized by the solvothermal method, and which was the key materials for the preparation of thin film luminescence sensor. The UCNPs can emit red, green and blue three basic color under the excitation of 980 nm laser. The fluorescence can be controlled by doping the rare earth ions.(2) Thin film luminescence sensor has been successfully made. The sensor was made by screen printing film and microwave heating film. Flexible base was added so that the film can be united to porous acrylic plate closely and nondestructive. Therefore, the array thin film luminescence sensor with uniform size、 controllable morphology and intense fluorescence was prepared.(3) The qualitative detection of thin film luminescence sensor is realized. Based on the basic principle of Fluorescence resonance energy transfer(FRET), the sensing system is constructed, and the fluorescence signal is released by the naked eye, which achieved the qualitative detection of RB and SF.(4) The quantitative detection of thin film luminescence sensor is realized. Based on FRET, the spectral data of sensor system was measured by fluorescence spectrometer. The integral intensity ratio(IIRRG) of the red bands in RB and the green bands in UCNPs were calculated. By constructing the function model of the IIRRG and the concentration of RB, the corresponding value of IIRRG to RB will be found, so as to achieve the quantitative detection of RB.