Research On Cell Manipulation Based On Double-probe Nanorobot

In 1985, Gerd Binnig introduced the atomic force microscope(AFM) to the world for the first time, since then, AFM became an important tool in the field of nano science. Even today, people combine nanoscience with biomedicine. AFM in biomedical field serves as a research tool and it also promotes the development of biomedicine. Living cells can be detected and manipulated accurately under both of vapor and liquid conditions by AFM.AFM provides the effective method of researching cells in biomedicine field.Based on the research of the mechanical properties of cells, this thesis discussed the new method which disrupts cells by AFM. On the basis of the principle of AFM, the secondary development of relative program of double-probe nanometer robot and cell manipulation as well as the work of data processing is performed. The double-probe nanometer robot system is equipped with two probes which can work independently, the system formed the ‘nanometer tweezers' which is able to clip cells and it is able to be manipulated when put cells into liquid condition. Since the double-probe nanometer robot system has the ability to measure samples accurately. The dynamometry precision of nano-tweezer is able to reach the NP level. Compared with other methods which disrupt the cytomembrane, this system is able to pull the cytomembrane mechanically, accurately and directly, furthermore, it can disrupt the cytomembrane without through the methods of chemistry and biology, increasing the work efficiency of cell-breaking effectively.