Role Of Alpha-enolase In Hepatitis B Vigils Replication And Its Possible Mechanism Of Action

Objective:Persistent chronic infection with hepatitis B virus(HBV)is a major risk factor for the development of HBV-related diseases.The molecular mechanisms that underlie HBV infection and replicat ion are not fully understood.The aim of this study was to explore the role of ENO1 in HBV replication processes.Methods:we examined ENO1 expression levels in HBV-infected and non-HBV-infected liver tissues and cells by Western blot anal ysis,real-time PCR and immunohistochemistry.In addition,HBs Ag and HBe Ag in the media of transfected Hep G2.2.15 cells were detected using an elec trochemical luminescence anal yser within 48 hours after ENO1-specificsi RNA transfection.The expression levels of HBV DNA,type I interferon and 5 downstream IFN-stimulated genes in Hep G2.2.15 cells were examined using real-time PCR.Results:We found ENO1 expression was upregulated in the HBV-infected liver tissues and cells.Silencing of ENO1 resulted in a significant reduction in HBV replication,and this si RNA-mediated reaction also caused the upregulation of expression of type I interferon and downstrea m IFN-stimulated genes.Conclusion: we come to the conclusion ENO1 is involved in HBV replication.It is therefore likel y that HBV replication is enhanced following suppression of the IFN signalling pathway.However,the mechanisms that underpin ENO1-mediated modulation of the IFN signalling pathway remain to be elucidated.