Study On Active Substances Of Anti HepG2 And Hela Cell And Their Action Mechanism

Objective The CCK-8 method was used to screen the anti-human hepatoma Hep G2 cells and Hela cells of cervical cancer samples obtained from the samples isolated from plants,and then the anti-tumor mechanism of active samples was preliminary explored to develop antitumor drugs and lay the foundation.Methods Determined by CCK-8 assay to detect different concentrations 5-FU role of human hepatoma Hep G2 cells and human cervical carcinoma Hela cells,and get the best concentration of action.Using this concentration of 5-FU as a positive control,the activity of anti-human hepatoma Hep G2 cells and human cervical cancer Hela cells was screened for some samples from plants;Apoptosis of Hep G2 cells and Hela cells was tested by using 33342/PI double staining kit to detect the effects of Solasonine,Solasodine,Solamargine and Khasianine on Hep G2 cells and Hela cells.Real-time quantitative PCR was used to detect the expression of related apoptosis genes Bcl-2 and Bax in Hela cells and Hep G2 cells.Results The inhibitory rates of human hepatoma Hep G2 cells and human cervical cancer Hela cells at the concentration of 50 ?g/m L of 5-FU were 86.38±3.410 and 75.77±3.047.The monocrotaline and holmium monocrotaline are not high active substances in human hepatoma Hep G2 cells and human cervical cancer Hela cells.Solasonine,Solasodine,Solamargine and Khasianine have high activity against human hepatoma Hep G2 cells and human cervical cancer Hela cells.For Hep G2 cells,the IC50 were 17.70 ?g/m L,24.05 ?g/m L,19.89 ?g/m L,and 20.32 ?g/m L,respectively.For the He La cells,the IC50 was 26.18 ?g/m L,22.50 ?g/m L,22.44 ?g/m L,and 26.31 ?g/m L,respectively.The Hoechst 33342/PI double staining kit was used to detect the apoptosis of human hepatoma Hep G2 cells and human cervical cancer Hela cells after adding different concentrations of Solasonine,Solasodine,Solasonine,Solasodine,Solamargine and Khasianine.Real-time fluorescence quantification of related apoptosis genes Bcl-2 and Bax in Hela cells and Hep G2 cells.In Hep G2 cells,the positive group,Solasonine,Solasodine,Solamargine and Khasianine lowered Bc1-2 and raised Bax.In Hela cells,Bc1-2 was down-regulated in the positive group,Solasonine,Solasodine and Solamargine.The Bax was up-regulated in the positive group,Solasonine and Khasianine.Conclusions Solasonine,Solasodine,Solamargine and Khasianine all significantly inhibited the growth of human hepatoma Hep G2 cells and human cervical cancer Hela cells,and had a certain relationship with the concentration;Solasonine,Solasodine,Solamargine and Khasianine can induce apoptosis of Hep G2 cells and Hela cells.Through the detection of apoptotic genes Bax and Bcl-2,it was proved that Solasonine,Solasodine and Solamargine eare available.Solasonine,Solasodine,Solamargine and Khasianine may induce apoptosis of Hep G2 cells through down-regulation of Bc1-2 and up-regulation of Bax;Solasonine,Solasodine and Solamargine may be induced by apoptosis of Hela cells through down-regulation of Bc1-2;Solasonine and Khasianine may induce apoptosis in Hela cells by up-regulating Bax.