| Background:Myasthenia gravis is a class of autoimmune disease mainly involving the neuromuscular junction postsynaptic membrane.Although so far the pathogenesis of myasthenia gravis has not yet fully understood,but after a lot of research suggests that MG results from abnormal thymus,prouduces acetylcholinereceptor antibody,have been broad consensus.These antibodies are dependent on the proliferation and differentiation of B lymphocytes,and this process involves the participation of a variety of cytokines,at the same time.Chemotactic factor is a group of protein,consisting of polypeptides with low molecular weight(mostly 8-10kDa).CXCL13 is a kind of Chemotatic factors,which play an important role in the immune response,also known as B cell chemokine binding CXCR5,induced B cell activation,chemotaxis and migration of B cells to produce antibodies targeting.It is reported that CXCL13 plays a important role in several antoimmune diseases,and it is reported that CXCL13 is high expression of serum and hyperplastic thymus tissue in patients with myasthenia gravis.According to statistics,10% to 20% of patients with MG complicated with thymoma,and 30% to 50% of patients with thymoma complicated with myasthenia gravis.Patients with myasthenia gravis complicated with thymoma thymus and thymoma resection,the clinical symptoms of myasthenia gravis eased or even disappear accounts for 70%.This study aims to contrast CXCL13/CXCR5 expression in different tissue,to explore the relationship between thymoma with myasthenia gravis.Objective:The purpose of this study is to explore the meaning of CXCL13 chemotactic factor and its receptors CXCR5 on the occurrence of normal Thymus,hyperplastic thymus,thymoma and its para tumor tissue complicated with myasthenia gravis,and investigate the role of CXCL13/CXCR5 in the pathogenesis of myasthenia gravis and the correlation between thymoma and myasthenia gravis.Methods: Patients with MG complicated with thymic hyperplasia(40)and thymoma(30)were respectively collected in The first hospital of Shijiazhuang.And normal thymic tissue(24)in patients with heart disease was also collected.Resected tissue samples are examined by Quantitative Real-time PCR to obsever the expression level of CXCL13/CXCR5 mRNA.The data were expressed by(median,interquartile range),and SPSS 17.0software was used for statistical analysis.Each group was compared with One-way analysis of variance,and a value of P<0.05 was considered statistically significant.Result:1 The expression levels of CXCL13/CXCR5 mRNA in the tissues of patients with myasthenia gravis were higher than that of control groups(P<0.05),2 The expression levels of CXCL13/CXCR5 mRNA in thymoma tissues complicated with MG patients were higher than para tumor tissue(P<0.05),3 The mRNA expression level of CXCL13/CXCR5 has no difference during the para tumor tissue and hyperplastic thymic tissue(P>0.05).Conclusion:1 The expression levels of CXCL13/CXCR5 mRNA in the tissues of patients with myasthenia gravis were higher than those in controls,suggesting that CXCL13 and CXCR5 are of great significance in the pathogenesis of myasthenia gravis2 The expression levels of CXCL13/CXCR5 mRNA especially higher in thymoma tissue,but there was no statistical difference between the expression in para tumor tissue and hyperplastic thymic tissue,indicating that thymoma plays an important role in the pathogenesis of myasthenia gravis3 CXCL13/CXCR5 may become the new targets for the treatment of myasth-enia gravis. |
