The Role Of Eukaryotic Elongation Factor 2 (eEF2) In Myocardial Ischemia And Reperfusion Injury

Objective: This study is to determine the expression and location of eukaryotic elongation factor 2(eEF2)and the SUMOylation of eEF2 in cardiomyocytes during myocardial ischemia and reperfusion injury(MIRI).In addition,we investigated whether such changes could lead to some effects on myocardium in MIRI.Methods: We blocked the left coronary artery(LCA)to induce MIR in SD rattus,confirmed by ST-elevation and Evan’s blue/TTC staining.Hypoxia/reoxygenation(H/R)was treated with H9c2 cells.And it is western blot and some immune related staining we used to reflect expression of eEF2,phosphorylated eEF2,SUMO,Bax and Bcl-2.Some flow cytometry tests were conducted to uncover the influence of eEF2 and other 3 fragments.Results: Phosphorylated eEF2 to eEF2 ratio decreased during MIRI,and phosphorylated eEF2 translocated to nucleus during reperfusion phase through the assistance of SUMOylation.And more experiments in vitro indicated that eEF2 could inhibit apoptosis in this model.While,after truncation of eEF2 plasmid,N-terminal fragment could result in translocation and promoting apoptosis.Conclusions: eEF2 could have some dual effects on MIRI,and it might be related to SUMOylation and nucleus translocation.