Changes And Significance Of S100A1,Lactate Dehydrogenase And Creatine Kinase Isoenzymes In Infarcted Myocardium

Objective To investigate the changes and significance of S100A1,lactate dehydrogenase and creatine kinase isoenzymes in infarcted myocardiumMethod 1.92 SD male rats by ligation of the left anterior descending coronary artery were randomly divided into normal control group,sham operation group,myocardial ischemia 15 min,30min,1H,3h,6h,9h group.HE staining,Masson staining,immunohistochemistry,immunofluorescence,ELISA,Western blotting were used to detect the expression of S100A1 and CKMB,LDH groups in myocardial tissueand and in the blood.2.30 cases of autopsy cases were divided into non infarcted group,suspicious infarction group and myocardial infarction group.Immunohistochemistry and Western blotting were used to detect the expression of S100A1 and CKMB and LDH in myocardium.Results 1.Animal experiment results: HE staining and Masson staining showed light staining of myocardium under light microscopy at 9 h after ligation;CKMB immunofluorescence results showed that CKMB expression was normal in both CON and Sham groups,but significantly decreased from 1 hour after coronary artery ligation,until 9 h,there is a large number of expressions missing.Immunohistochemistry showed that the positive expression of CKMB in sham-operated group was slightly decreased compared with CON,but decreased from30 minutes to 3 hours after coronary artery ligation,and decreased more significantly after 6hours,and there was a significant difference compared with CON group(P <0.05).The detectionof CKMB expression in serum showed a slight increase in CKMB at 15 min after coronary artery ligation and a significant increase after 6 h(P < 0.05);LDH immunofluorescence showed normal expression in both CON and Sham groups,and from coronary artery ligation At the beginning of30 minutes,it weakened obviously.With the increase of coronary artery ligation time,the fluorescence intensity gradually weakened until 9 hours,and there was a large loss of expression.LDH expression began to decline when coronary artery was ligated for 15 minutes,and it could be seen that the myocardial ischemia decreased significantly after ligation of ischemia for 1 hour.With the increase of myocardial ischemia time,the overall level of LDH showed a downward trend,and the expression of LDH at different time points.Compared with the CON group,there was a statistically significant difference(P < 0.05).LDH in the serum increased at 15 min after coronary artery ligation,and increased significantly at 3 h after ligation of myocardial ischemia,and tended to be highly stable at 6 h and 9 h.The expression of LDH at each time point was statistically significant compared with that of CON(P < 0.05).Immunofluorescence and immunohistochemistry results of S100A1 showed that the expression level of S100A1 in Sham group was not significantly different from that of CON group.There was a significant decrease in the expression of coronary artery at 15 minutes after ligation(P < 0.05).The expression of S100A1 reached a low peak at 6 h after coronary artery ligation,and a clear downward trend can be seen with myocardial deficiency.With the increase of blood time,the overall level of S100A1 showed a downward trend.Serum concentrations of S100A1 in the coronary artery ligation 15 min increased,and increased significantly at 3 h after coronary artery occlusion.At the subsequent 6 h and 9 h time points,the concentration of S100A1 continued to increase,showing an overall increase trend.Compared with CON group,the concentration of S100A1 in plasma increased significantly in each group(P < 0.05).Western blotting showed that the LDH protein expression levels were basically flat at CON,Sham,15 min,began to decline at 30 min,andsignificantly decreased at 6h.The protein expression of CKMB began to decrease at 15 min,and reached the maximum level in the experimental group at 3 h,but then the expression increased.The overall infarction group was lower than the CON and Sham groups.The expression of S100A1 was down-regulated at 15 minutes after coronary artery ligation,significantly down-regulated at 30 minutes,and decreased overall.All of the above-mentioned differences were statistically significant(P<0.05).2.Human myocardial tissue experimental results:In the non-infarction group,the coronary arteries and myocardium had no pathological changes;in the myocardial infarction group and the suspicious infarction group;the coronary lumen stenosis was more than 50%,and the suspicious infarction group had the basis of infarction but no histological changes of the infarction.Immunohistochemistry and Western blotting showed that the optical density of S100A1,LDH,and CKMB staining in the myocardial infarction group was significantly lower than that in the non-infarct group(P<0.05).The optical density of S100A1 and CKMB in the suspicious infarction group was also lower than that in the non-infarct group.In the group(P<0.05),S100A1 and LDH were still higher than those in the myocardial infarction group(P<0.05).Conclusion 1.The expression level of S100A1,LDH and CKMB in myocardial tissue decreased with the increase of myocardial infarction time.3.Detection of myocardial cell S100A1,LDH,and CKMB expression may contribute to the diagnosis of acute myocardial infarction.