The Research Of The Interaction Between LncRNA(SOX2OT)and PCGF1 In Glioma Cells

Objective:The polycomb protein family played a crucial role in the development and progression of gliomas as transcriptional repressors via epigenetic changes.Some studies showed that long-chain non-coding RNAs（IncRNAs）were abnormally expressed in gliomas.The abnormal IncRNAs expression was associated with the tumor development,proliferation,migration and invasion.It played a key role in the tumor pathophysiology changes and prognosis.In this study,we demonstrated the expression of IncRNAs in induced differentiated malignant glioma U87 cells.The expression changes of candidate interfering IncRNAs were evaluated after up-regulation and down-regulation of polycomb group ring finger 1（PCGF1）.We demonstrated the relationship between PCGF1 and IncRNAs in gliomas and identified the candidate IncRNAs interacted with PCGF1 in glioma U87cells.Method:First of all,the expressions of PCGF1 and the lnc RNAs selected which may interact with PCGF1 were detected by Real-time quantitative PCR in malignant glioma cells such as H4,U251,U87 cells;Then U87 glioma cells were induced to differentiation with 10^-6mol/L concentration of retinoic acid.The expressions of PCGF1 and candidate lncRNAs were detected in several differentiation stage.Moreover,the expressions of candidate lncRNAs were tested after raising or knocking down PCGF1expression comparied with the expressions of candidates in normal U87 cell.Then,the functional interactions between lncRNAs and PCGF1 were identified by applying MTT assay and RNA binding protein Immuno precipitation（RIP）.Result:1 The expression of PCGF1 was positively related to the malignant degree in different glioma cell lines.The marked inequality expression among different lncRNAs exsited in glioma cell lines.2 In the retinoic acid induced differentiation process（0¹0days）of U87glioma cells,the expression level of PCGF1 decreased initially and achieves the lowest point at the sixth day;but rised in the following days till the tenth day;The expression of lncRNAs decreased in the differentiation stage.3 The expression levels of lncRNAs change after over-expressing or down-regulating the expression of PCGF1.What is more,the expression of SOX2OT significantly change.4 Knocking down the expression of SOX2OT inhibited the growth of U87 glioma cells and raised the ratio of cell apoptosis.5 The interaction between SOX2OT and PCGF1were identified by RIP essays.Conclusion:1 PCGF1 expresses in glioma and is closely relative to the malignant degree of gliomas.2 SOX2OT can promote the growh of U87 glioma cells and inhibit apoptosis of U87 glioma cells.3 PCGF1 interactes with SOX2OT.