Study On Capture And Release Of Circulating Tumor Cells Using Microfluidic Chip And Nanomaterials

People's life and health suffer from a serious threat of malignant tumors,due to the prevalence of unhealthy lifestyles,which may lead to the emergence and spread of malignant tumors.The incidence and mortality of cancer patients are extremely high.Obviously,the prevention and early treatment of cancer are very important for cancer patients.However,the sensitivity and specificity of conventional methods of cancer diagnosis are very low.When malignant tumors in patients are detected by medical devices,they are in terminal stage of cancer and miss the optimal treatment period.At present,medical researchers pay more and more attention to the research of CTCs,and made breakthroughs in the detection and analysis of CTCs.Circulating Tumor Cells(CTCs)are tumor cells that fall from the primary tumor and enter the peripheral blood circulation system.The study has shown that CTCs play an important role in cancer research of the diagnosis,treatment and monitoring of tumors.CTCs can be effectively used for early diagnosis in vitro,rapid evaluation of chemotherapy effects,and prognosis of surgery.Over the past decade,nanomaterials and microfluidic technologies have been able to control the micro-and nanoscale range and have gradually become effective detection methods for CTCs.So far,the defects of CTC detection technology is low in the capture efficiency and the purity.the effective release of high viability cannot be achieved,so that cell culture cannot be carried out.The microfluidic chip is used as an experimental platform to promote the miniaturization of complex biochemical experiments.It has the advantages of high integration,low sample volume,short experimental time,higher sensitivity and controllability.It is easy in observation and analysis,and portability.When the microchannel size and cell size of the microfluidic chip match relatively high,the cell population and even individual cells can be separated and analyzed.Microfluidic chip technology has been widely used in the biomedical field.Nanomaterials have superior performance and are widely used in medical,electrochemical and optical research fields.For example,in the field of medical biology,nanomaterials have good biocompatibility and are applied to the fabrication of biosensors,biomarkers,and drug delivery.With the sustainable development of the synthesis methods and characterization methods of nanomaterials,researchers have prepared nanomaterials of different shapes and sizes and stable performance.Nanomaterials have a wide range of good applications in catalytic applications,dye-sensitized solar thin-film batteries,super capacitors,and biochemical sensors.This paper makes conclusion that the research progress in the detection and analysis of CTCs.In the face of the problems existing in the separation and enrichment of CTCs,we have made great efforts in the detection and analysis of CTCs using the advantages of nanomaterials and microfluidic chips.The main research work is as follows:1.We designed a novel microfluidic chip that contains a tapered microchannel for capturing and releasing circulating tumor cells.It has a degradable zinc oxide nanorod substrate on its inner surface.2.We applied a Zn O thin film on the glass plate by magnetron sputtering.A mixture of aqueous solutions of zinc nitrate hexahydrate and hexamethyltetramine is prepared in a certain proportion.A layer of Zn O nanorods(approximately 2 ?m)was grown using water bath heating.3.Based on continuous size filtration and microfluidic chips for soluble ZnO substrates,we demonstrated efficient CTCs capture release capabilities,as well as downstream molecular analysis.Based on the size of the filtration,we studied the effects of different flow rates,blood samples with different cell lines on the capture efficiency of CTCs,and the microfluidic chip without substrate was used as a control group to study its related effects.Based on the soluble substrate,we studied the effect of different concentrations of dilute phosphoric acid on the release efficiency of CTCs and CTCs viability when the release time of CTCs was determined.We also set up a control group to study its related effects.CTCs achieve high cell viability through continuous culture for several days,providing a basis for downstream detection and analysis.4.We have designed an etching method for three-dimensional microstructures.The suspension of the micro-nanoparticles with inhibited etching is coated on the surface of the glass sheet,and the surface of the glass sheet is etched by the etching solution to obtain a glass sheet with several depressed microstructures on the surface.The glass sheet is made into a microfluidic chip based on size for efficient sorting and enrichment of CTCs.