| As one of the threats to the safety of human life,there are a large number of people dying of cancer every year in the world.Surgery,radiotherapy and chemotherapy are the traditional methods of cancer treatment.In recent years,with the rise of nanotechnology,the treatment and diagnosis of cancer can overcome the shortcomings of traditional methods.Through nanotechnology to nanoparticle synthesis for tumor diagnosis and treatment of one of the multifunctional nanoparticles can set targeting,imaging and treatment in one,and low toxicity,which makes the nanoparticles brings innovative irreplaceable in tumor therapy.One of the differences between the microenvironment of tumor cells and normal cells is that the former cell pH is larger than the latter,This paper constructs a series of new cancer reported to the multifunctional nano probe,and discusses the potential applications of these optical probe in the diagnosis and treatment,including the following three aspects of the work force;1,A new type of gold nanoparticles modified by amino acids was used to improve the selectivity of heavy metal ions detectionIn this chapter,the surface modification of gold nanoparticles was used to modify the concentration of arginine into different concentrations of mercury ions.First of all,we need to synthesize gold nanoparticles.The gold nanoparticles are nano sized complexes containing citric acid and arginine groups.When the concentration of mercury ion was 5umol/L,the color of the solution showed the deepest purple,and with the increase of the concentration gradient,the UV absorption was linear.27 the intensity of the sound signal can also be used to show the change of mercury ion concentration,which is the highlight of our work.After the modification of the gold nanoparticles by surface chemical reaction,the stability of the gold nanoparticles can be proved by the change of the particle size and the electric potential with the time.With the addition of heavy metals,the larger the color of the particles,the deeper the color.2,The construction of a total package of dye Cy5 molecules are very soft manganese oxide nano carrier for targeted and near infrared light driven precision therapyThis chapter uses the Cy5 and MnO by dye molecules covalently linked together,the targeting of Cy5 with tumor,MnO molecules with magnetic targeting to effect also has MRI function with targeted molecular imaging together can,Cy5 molecules can emit fluorescence in high quantum yield the laser excitation,so as to achieve the effect of multimodality imaging.3,To construct a novel acid sensitive nano carrier for photodynamic therapy of tumor cellsIn this chapter,we designed and synthesized several kinds of multifunctional nano probes.One of the nano diagnostics is the acid sensitive nanoparticles,the nano probe in the circulating process until the arrival of the location of the tumor fluorescence presents a "OFF to ON" change,hyaluronic acid by hydrophobic loaded second photosensitizer chlorin E6 two,due to the tumor cell surface strong acid,HA-ADH-Ce6 complex(HACE NPs),the compound by CD44(hyaluronic acid receptor on the surface of tumor cells)were lung cancer cell A549 cell rapid uptake,encounter acidic environment will automatically open and release Ce6 two chlorin E6 fluorescent molecular fluorescence,the laser irradiation in tumor 5 times the free Ce6 fluorescence emission,the photoacoustic intensity is 3 times more than the free Ce6.As the second generation photosensitizer,Ce6 can produce reactive oxygen species for photodynamic therapy.The second isIn the above work,no matter what kind of nano probe needs to first find the distance vector synthesis,good water,inorganic or organic dye molecules carrying through a combination of covalent chemical bond.The package,the EPR effect of tumor surface,for the synthesis of nanoparticles size should be in the range of 10-100 nm and the Electronegativity of nanoparticles in a suitable range,particle size and zeta potential with time change,nano probe fluorescence emission and good stability.The HA-ADH-Ce6 complexes were synthesized by self-assembly to form HACE NPs,in which the molecular weights of hyaluronic acid were 20,50 and 100 kDa,respectively.By electron microscopy of hyaluronic acid-two chlorin E6 nanoparticles were characterized.The particle size obtained is consistent with the results of particle size measurement.To test the effect of different molecular weight,HACE20 NPs of tumor therapy and photodynamic effect of fluorescence intensity than other nanoparticles to high molecular weight,HACE20 NPs is a good drug carrier,which can transport the anticancer drug doxorubicin can efficiently gather in the tumor site for NIR/PA dual mode imaging treatment of tumor.Cy5-MnO NPs second nano particles using chelating the dyes and inorganic magnetic chelating MnO molecule together,targeting tumor site and EPR effect of Cy5 dyes,nanoparticles can make rapid accumulation in the tumor site,also can realize double NIR/MRI image mediated antitumor effect,such it will greatly increase the accuracy of tumor therapy.The main objective of this chapter is the synthesis of multifunctional nanoparticles to achieve accurate treatment of tumors,tumor imaging and treatment in photoacoustic / fluorescence mediated by /MRI in the process of carrying through the nano preparation of chemotherapy anticancer drug doxorubicin then chemotherapy on tumor cells,improve the treatment effect of cancer.I am the first synthesis of nanoparticles for tumor targeting effect is very good,good hydrophilicity of hyaluronic acid carrier(HA)second generation photosensitizer chlorin E6 two loaded with photodynamic treatment effect to improve the destruction of tumor cells.The synthesized nanoparticles were rapidly expressed by CD44(the hyaluronic acid receptor on the surface of tumor cells)by A549 cells of lung cancer cells. |
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