| Acute myocardial infarction(MI)refers to acute coronary artery occlusion,sharp reduction of coronary artery or interrupt,make corresponding regional myocardial is severe and prolonged ischemia,hypoxia condition,is the world's third largest cause of death.Research is more,western medicine and interventional therapy and traditional Chinese medicine treatment of coronary heart disease research focuses on the method for activating blood circulation and related drugs.Found in our previous studies,Chinese medicine dogwood single element-morroniside,in rats with focal cerebral ischemia model,early has antioxidant and anti-inflammatory effects,resistance to apoptosis,the later can promote the endogenous neurogenesis and angiogenesis in ischemic edge.This study by discussing mono nucleoside dogwood single component extract on experimental myocardial ischemia reperfusion of myocardial protection in rats and its mechanism,proved from two aspects of antioxidant and antiapoptotic mechanisms,for further study on the late mono glycosides for myocardial infarction myocardial regeneration and the role of angiogenesis,as well as the clinical use of mono glycosides treatment of myocardial infarction,to provide experimental basis.Choose 6~8 weeks of male SD rats,by coronary ligation method to establish myocardial ischemia model.Postoperative were divided into control group,model group,morroniside low-dose group(45 mg/kg),morroniside medium-dose group medium(90 mg/kg),morroniside high-dose group(180 mg/kg),the treatment group and positive control group.3 h after lavage,3 days in a row,control group and model group to the amount of normal saline.After the observation,part of the big abdomen aortic blood detection of serum MDA,SOD and LDH,and cTnT levels,parallel TTC staining detection infarction volume.Another part of the fresh materials and perfusion materials to return heart specimens respectively,TUNEL staining to observe apoptosis edge area of ischemia,western blot method of semi-quantitative analysis of the expression of apoptosis of ischemic myocardial tissue factor.Results:(1)after TTC staining,quantitative analysis shows that,compared with model group,only mono nucleoside high-dose treatment group can significantly reduce myocardial infarction size(P < 0.05).(2)the serum biochemical indicators: serum detection,according to the results than with the control group,model group significantly increased contents of LDH and cTnT(P < 0.001,P < 0.001);Compared with model group,the mono glycosides LDH content in small,medium and large dose group were decreased significantly(P < 0.001),mono glycosides,high-dose group of cTnT content decreased significantly(P < 0.05,P < 0.01).(3)biochemical indicators: tissue homogenate test results showed that MDA content than with the control group,model group significantly increased(P < 0.001),SOD content decreased significantly(P < 0.05);Compared with model group,the mono glycosides high-dose group of MDA content decreased significantly(P < 0.05),mono glycosides,high-dose group of SOD activity was significantly increased(P < 0.05,P < 0.01).(4)Apoptosis factors: western blot test showed that compared with the control group,model group the Bcl-2 expression significantly reduced(P < 0.001),mono glycosides expression in each treatment group were significantly higher(P < 0.05,P < 0.001 and P < 0.001);And bax,caspase 3,caspase-8 after building expression were significantly increased compared with control group(all P < 0.001),mono glycosides treatment,groups of bax expression were significantly lower(all P < 0.001),only in the dose group and high dose group of caspase 3 significantly lower(all P < 0.001),caspase-8 only expressed in large dose group decreased significantly(P < 0.01).In conclusion: mono glycosides can significantly reduce the myocardial infarction area,effectively protect the ischemic tissue.It can function in two ways: one is that increase the activity of SOD in tissue,reduce the production of MDA,reduce tissue oxidative stress damage;The second is that it can reduce ischemic myocardial apoptosis factors Bax,caspase-3,the expression of caspase-8,increase the expression of Bcl-2,inhibiting cell apoptosis. |
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