Effect Of SiRNA Silencing LMO4 Gene On The Growth Of Human Gallbladder Carcinoma Cell Line

Objective:Gallbladder cancer(GBC)is the most common malignant and fatal tumor of the biliary tract.Diagnostic and prognostic markers for this malignancy have not been extensively studied,and,due to lack of conspicuous symptoms and physical signs,the majority of patients are diagnosed at an advanced and incurable stage.Moreover,GBC is resistant to chemotherapy or radiotherapy,surgical resection is the only potentially effective treatment for GBC.LMO4 is a novel member of the LIM-only(LMO)subfamily of LIM domain-containing transcription factors,so named because they are composed almost entirely of two tandem LIM domains.They all play important roles in the normal mammalian development,functioning as an important regulator of cell proliferation.LMO4 is highly expressed in the epithelial compartments at locations of active epithelial mesenchymal interactions,and can interact with some signaling pathways involved in epithelial mesenchymal signaling.The expression of lmo4 in the nucleus may be an independent predictor of the prognosis of breast,pancreatic and tongue cancer.However,there is no evidence of lmo4 in the study of gallbladder cancer cells so far.The aim of this study was to investigate the effect of siRNA targeting lmo4 on the growth of humangallbladder carcinoma OCUG and NOZ cell line,and to provide experimental data and support for lmo4 as an effective biomarker and therapeutic target for gallbladder carcinoma.Methods:In this study,the effect of siRNA on the proliferation and apoptosis of OCUG and NOZ cell line was observed by reducing the expression of LMO4 gene.RT-PCR and Western blot were used to detect the effect of siRNA transfection on the expression of LMO4 in middle OCUG and NOZ cell line.Cck-8 cell count and colony formation assay were used to observe the proliferation rate of OCUG and NOZ cell line after siRNA transfection.Results:The results of RT-PCR and Western blot showed that the expression of LMO4 in OCUG and NOZ cell line was inhibited effectively after transfection of siRNA.The results of cck-8 cell count and colony formation showed that the proliferation of OCUG and NOZ cell lines could be effectively inhibited by transfection of siRNA.Conclusion:Expression of LMO4 is related to proliferation and differentiation of gallbladder carcinoma.LMO4 levels predict prognosis in patients with gallbladder cancer,especially those with high proliferation.This study has increased our understanding of the role of LMO4 in the proliferation of gallbladder cancer and may provide new molecular targets for the treatment of clinical gallbladder cancer patients.