The Role Of Long Noncoding RNA LOC101928505 In The Tumorigenesis And Development Of Pancreatic Cancer

Background: Long noncoding RNAs(lnc RNAs)are non-protein coding RNAs,and longer than 200 nucleotides in length.In recent years,many studies have reported that lnc RNAs can regulate gene expression via different molecular mechanisms,including chromatin modification,epigenetics,genomic imprinting,and regulation of protein activity.Many evidences have found that long non-coding RNAs such as HOTAIR,MALAT1,CRNDE,and GAS5 have are abnormally expressed in pancreatic cancer tissues and cells,and are closely related to the development of pancreatic cancer,and may be a marker for early diagnosis of pancreatic cancer and Biotherapy targets.Objective: This study was aimed to screen for long-chain non-coding RNAs differentially expressed in pancreatic cancer tissues and adjacent normal tissues,and to investigate the effect of regulating expression levels of pancreatic cancer cells on proliferation,apoptosis,and migration of pancreatic cancer cells in vitro.And the role of long non-coding RNA in the development of pancreatic cancer was initially elucidated.Methods:(1)In this study,we try to detect the differential expression of lnc RNAs in three groups of pancreatic cancer and adjacent normal tissues by using lnc RNA expression microarray,and to screen lnc RNA LOC101928505;RT-PCR was used to detect the expression of lnc RNA LOC101928505 in 20 pairs of pancreatic cancer and adjacent normal pancreatic tissues.(2)After construction of lnc RNA LOC101928505 sh RNA knockout plasmid and pc DNA3.1 overexpression plasmid,we used the CCK8,flow cytometry and Transwell experiments to explore the effects of lnc RNA LOC101928505 expression on malignant biological behavior of pancreatic cancer such as cell proliferation,apoptosis and invasiveness.Results:(1)We used lnc RNA expression microarray to detect the differential expression of long non-coding RNAs in three pairs of pancreatic cancer and adjacent normal tissues,the results found an abnormally high expression of long non-coding RNA LOC101928505.Then quantitative RT-PCR was performed on 20 pairs of pancreatic cancer and para-cancerous pancreatic tissue,and the results confirmed that the expression level of lnc RNA LOC101928505 in pancreatic cancer was significantly higher than that of para-cancerous pancreatic tissue(P<0.05).(2)The shRNA knockout plasmid and the pc DNA3.1 overexpression plasmid of lnc RNA LOC101928505 were successfully constructed,and the pancreatic cancer cells with stably expressing the above plasmid were selected.Our data of CCK-8 detection found that down-regulation of LOC101928505 could decrease pancreatic cancer cell proliferation(P<0.05);Our data of flow cytometry showed that the early apoptotic cells in all pancreatic cancer cells with down-regulation of LOC101928505 were significantly higher than those in the control group(P<0.05).And transwell assay results showed that the invasion of pancreatic cancer cells with down-regulation of LOC101928505 was decreased(P<0.05).In addition,by up-regulating the expression level of LOC101928505 in Bx PC-3 and Panc-1 cells,CCK-8,flow cytometry,and Transwell experiments were used to find that the up-regulation of the expression of LOC101928505 in pancreatic cancer cells significantly promoted pancreatic cancer cell proliferation,inhibited cells Apoptosis and enhanced the invasion of pancreatic cancer cells in vitro.Conclusions: The study found that the expression of long non-coding RNA LOC101928505 in pancreatic cancer tissues was significantly higher than that in para-tumor normal pancreatic tissues.The abnormally high expression of LOC101928505 may promote the cell proliferation,inhibit cell apoptosis,and enhance the cell invasion in vitro in pancreatic cancer.It was initially confirmed that the long non-coding RNA LOC101928505 plays the role of an oncogene in the development of pancreatic cancer.