The Clinical Significance Of MiR-93-5p In Gastric Cancer And Its Underlie Molecular Mechanism In Promoting Gastric Cancer Progression

Objective:To investigate the biological function and clinical significance of miR-93-5p in gastric cancer(GC),discovery the relationship between miR-93-5p and Hippo pathway,and find a new target for the treatment of GC.Method:1.Quantitative Real-time PCR(qRT-PCR)was used to detect the mRNA expression of miR-93-5p,FAT4 and LATS2 in 30 cases of fresh GC tissue and 30 cases of noncancerous tissues,and analysed the relation between the expression of miR-93-5p and the expression of FAT4 and LATS2;then analyzed the relationship between the expression of miR-93-5p and clinical pathological features in gastric cancer.2.The miR-93-5p mimics and NC were transfected into SGC-7901,and the miR-93-5p inhibitor and inhibitor NC into HGC-27 cells when they reached 50–60% confluence,according to the manufacturer's instructions.3.Western blot was used to detect the expression of essential components of the Hippo pathway,the position of YAP in GC cells and the expression of EMT protein in GC cells after transfection4.A CCK-8 assay and colony formation assay was conducted to evaluate cell proliferation.The SGC-7901 and HGC-27 cells were transfected with miR-93-5p mimic or inhibitor,and seeded in 96-well plates(2000 cells/well).5.Transwell assay was conducted to detect the ability of migration and invasion in GC cells after transfection.6.Dual-luciferase reporter assay was conducted to detect the target of miR-93-5p.Results:1.The expression of mi R-93-5p significantly associated with tumor differenti-ation and lymph node metastasis status.2.MiR-93-5p is overexpressed in GC tissues and promotes GC-cell proliferation and chemoresistance in vitro.3.MiR-93-5p promotes GC-cell migration,invasion,and EMT in vitro.4.MiR-93-5p regulates multiple components of the Hippo-YAP signaling pathway.5.FAT4 and LATS2 are direct miR-93-5p targets.Conclusion:The results of the present study indicate that miR-93-5p exerts an oncogenic function during GC development,via direct binding to the 3'-UTR of FAT4 and LATS2,thereby inactivating the Hippo pathway.Understanding the mechanism underlying miR-93-5p dysregulation during GC progression will be essential to enable the development of new GC therapeutic strategies in the future.