| Objective:To study CD 10 expression of cancer associated fibroblasts(CAFs)and Cx43 expression of colorectal cancer cells in human colorectal cancer specimens and the effect on colorectal eancer initiation and progression.Methods:226 specimens of colorectal cancer surgical removal were collected in our hospital,which patients had been followed up.A series of immunohistochemical markers(CD 10,Cx43,Ki-67,P53,CyclinD1,β-Catenin,CD68,CD3,CD4,CD8)were applied on central zone of colorectal cancer tissues,the adjacent adenoma tissues and normal colorectal mucosa respectively by Envision method,observing expression of CD 10 in CAFs and Cx43 in colorectal cancer cells.Also their relationships with several clinic pathological parameters,tumor cells proliferation and mutation were studied.Results:CD 10 expression was not detected in the normal colorectal mucosa by immunohistochemistry.The percentages of CD 10 + CAFs were 80.1%(181/226)and 58.4%(132/226)respectively in central zone of colorectal cancer tissues and in adenoma zone adjacent to colorectal cancer tissues.SMA was also positive in CD10+area while Desmin was negative.High expression of CD 10 in CAFs was related with female,age.>50,infiltrate deep muscle layer and whole layer,middle and low differentiation of tumor,metastasis in lymph node and cancerous emboli(P<0.05).The survival rate of positive CD 10 in CAFs was higher than that negative one(P<0.05).Ki-67 labelling showed 84.1%(190/226)in central zone,63.7%(144/226)in adenoma zone respectively,while 7.1%(16/226)in the normal mucosa.The percentage of P53 was 50.4%(114/226)in central zone,53,1%(120/226)in adenoma zone respectively,while 8.8%(20/226)in the normal mucosa.The percentage of CyclinDl was 83.6%(189/226)in central zone,48.7%(110/226)in adenoma zone respectively,while no expression in the normal mucosa.The percentages of β-Catenin in normal intestinal mucosa,paracancerous adenoma area,central region of colorectal cancer was 100%(226/226),53.1%(120/226)and 17.7%(40/226)in cell membrane,0%(0/226),95.6%(216/226),100%(226/226)in cytoplasm,and 0%(0/226),10.6%(24/226),49.1%(111/226)in nucleus respectively.CAFs CD10 is positively correlated with Ki-67,P53,CyclinD1,β-Catenin in tumor cells and CD68 in interstitial cells(P<0.05),while CD3,CD4 and CD8 in stroma cells were not associated with CD 10+ CAFs(P<0.05).The percentage of Cx43 in central region of colorectal cancer was 64.2%(145/226)in nucleus,23.0%(52/226)in cytoplasm,0.8%(2/226)in cell membrane.And that in paracancerous adenoma area was 42.0%(95/226)in nucleus,65.9%(149/226)in cytoplasm,1.3%(3/226)in cell membrane.The Cx43 in normal intestinal mucosa was expressed in cell membrane and cytoplasm,no expression in cell nucleus.The Cx43 was related with male,invasive depth and metastasis in lymph node and cancerous emboli(P<0.05),which was no related with age and differentiation degree(P<0.05).Survival rates in Cx43 positive group were lower than that in Cx43 negative group.Cx43 in tumor cells is positively correlated with Ki-67,β-Catenin in tumor cells and CD68,CD3,CD4 in interstitial cells(P<0.05),while P53,CyclinD1 in tumor cells and CD8 in interstitial cells were not associated with Cx43 in tumor cells(P<0.05).Conclusion:CD 10 may be one new maker for CAFs,because it is more distinctive than SMA.CAFs with the high expression of CD 10 indicates the patient with poor prognosis by which tumor cell proliferation,mutation,metastasis and proliferation can be caused.Nucleus Cx43 in colorectal cancer cells with high expression can also indicate one patient with poor recover,by which tumor cell proliferation,invasion and metastasis.Nucleus Cx43 with high expression in colorectal cancer cells infiltration front can show increasing invasion ability of front cancer cells.CD 10 in CAFs and nucleus Cx43 in colorectal cancer cells promote Wnt signal pathway activation.CD 10 in CAFs and nucleus Cx43 in colorectal cancer cells were positive correlation,which lead to colorectal cancer progression together. |
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