Detection Of Circulating Tumor Cells In Patients With Breast Cancer Using Negative Enrichment Of Immune Magnetic Beads And The Study Of Its Clinical Significance

Backgroud::Detection of circulating tumor cells(CTC)as a new detection method has broad application prospects in clinical practice,this study detect the circulating tumor cells in patients with breast cancer by negative enrichment of immune magnetic beads to discuss the correlation between CTC and molecular subtypes and clinical characteristics of breast cancer.METHODS:152 breast cancer patients from August 18,2015 and February 20,2016who accepted CTC detection were studied.Negative enrichment of immune magnetic beads and fluorescence in situ hybridization(FISH)were applied to collect the CD45-/CEP+/DAPI+of CTC,the correlation between CTC and molecular subtypes and clinical characteristics of breast cancer were analyzed.RESULTS:In 152 patients with stage I to II,III and IV,the positive rates of CTC were 41.5%,60.0%,73.9%respectively,the difference is significant(?~2=9.360,P=0.009).There was no significant difference between CTC and molecular subtypes of breast cancer(P>0.05).The presence of CTC was uncorrelated with patients'T stage,N stage,hormone receptor and HER2 expression(P>0.05).In addition CTC was correlated with M stage(?~2=5.863,P=0.015).CTC has a close relationship with the patient's PFS,P<0.05.CONCLUSIONS:CTC was uncorrelated with molecular subtypes of breast cancer,but closely correlated with TNM stage and the occurrence of metastasis.The detection of CTC is conducive to more accurate assessment of the disease.