Zinc Phthalocyanine Encapsulated In Polymer Micelles Enhance Photodynamic Therapy On Osteosarcoma Treatment Induced By G2/M Cell Cycle Arrest

Zinc phthalocyanin(ZnPc),one of the second-generation photosensitizers,has been wildly used in photodynamic therapy.However,the poor dissolubility of ZnPc in water causes the low efficiency of drug delivery and reduces the cytotoxicity of photodynamic therapy(PDT)on tumor treatment.Thus,we employed a novel amphiphilic block copolymer poly(ethylene glycol)-poly[2-(methylacryloyl)ethylnicotinate](PEG-PMAN)to synthesize the ZnPc nanoparticle.MTS assay and Annexin V-PE/7-aad flow cytometry assay were employed to detect the apoptosis rate of OS cells induced by nano ZnPc particles.Then we detected the cell cycle alteration of PDT using flow cytometry assay and Western Blot.The formed PEG-PMAN/ZnPc nanoparticle(PPZ)dramatically increases apoptosis in osteosarcoma cells after light irradiation and promotes cell cycle arrest at G2/M.The excellent therapeutic effectiveness and safety of PPZ are also proved by in vivo experiments operating on osteosarcoma model.The finding above indicates PPZ has promising clinical applications as a next-generation photosensitizer in PDT of osteosarcoma.