Genetic Polymorphisms Of Toll-like Receptor 3 In Neonates With Respiratory Syncytial Virus Infection

Research Background:Respiratory syncytial virus(RSV)is a single minus-strand RNA virus belonging to the genus Paramyxovirus and belongs to the major viral pathogen of viral lower respiratory tract infections in infants and young children.RSV infiltrates infants and young children's respiratory epithelium and proliferate in large quantities,inducing host expression and secretion of a large number of proinflammatory cytokines and immune regulatory factors,while a large number of active substances will induce pneumonia,bronchiolitis,wheezing and infant asthma and other diseases happened.Previous studies mainly focused on the relationship between the virus and the body's oxidative stress,but it is rare for the study of oxidative stress-mediated Toll-like receptors.Toll-like receptors(TLRs)are a type I transmembrane protein those are a type of pattern recognition receptor those are also a natural receptor for the immune system to fight infection.Studies have shown that TLR3(Toll-like receptor 3,TLR3)can specifically recognize the virus dsRNA,so that the activation of cells and signaling pathways involved in the activation of respiratory epithelial cells,thereby mediating the occurrence of inflammatory reactions.In recent years,studies have shown that TLR3 plays an important role in antiviral immune response,but its relationship with respiratory syncytial virus infection is not clear yet.In this study,we research TLR3 geners3775290,rs3775291,rs3775292,rs3775296,rs13126816,rs5743312 a total of six gene polymorphisms in order to explore the relationship between TLR3 gene polymorphisms and neonatal respiratory syncytial virus infection susceptibility.Purpose:To investigate the relationship between TLR3 rs3775290,rs3775291,rs3775292,rs3775296,rs13126816,rs5743312 polymorphisms and neonatal respiratory syncytial virus infection and to study the genetic predisposition of RSV.Method:All the study subjects from September 2013 to May 2014 in Children's Hospital of Zhejiang University School of Medicine neonatal ward admitted to hospitalized children with pneumonia,of which 163 cases of neonatal RSV infection in children with pneumonia as a case group,151 cases of newborns Non-RSV pneumonia children as a control group,collected during hospitalization for all children with blood samples and blood samples were extracted genomic DNA,the use of polymerase chain reaction(PCR)detection of TLR3 gene rs3775290,rs3775291,rs3775292,rs3775296,rs13126816,rs5743312 loci polymorphism,using SNPscanTM technology to TLR3 gene loci at various genotypes and allele frequencies,and statistical analysis.Result:The genotype and allele frequencies of TLR3 rs3775290,rs3775291,rs3775292,rs3775296,rs13126816 and rs5743312 were not significantly different between the case group and the control group(P>0.05).Conclusion:Polymorphisms of rs3775290,rs3775291,rs3775292,rs3775296,rs13126816 and rs5743312 of TLR3 gene may not be associated with the incidence of respiratory syncytial virus infection in neonates.