MiR-1-3p Suppresses Biological Functions Of Cancer Cells Via Regulating PD-L1 Expression In Hepatocellular Carcinoma

Objective:To investigated the tumor gene miR-1-3p surpress hepatoma development and progression though targeting PD-L1.Methods:?.miR-1-3p suppresses the PD-L1 expression1.Cells were infected with miR-1-3p overexpression lentivirus(miR-1-3p)and measured their transfection efficiency by q RT-PCR.Cells with stable overexpression of miR-1-3p were selected by flow cytometry.2.The sequence and the possible targets of miR-1-3p were predicted by the online software RNA-hybridization3.The expression of miR-1-3p in the group of miR-1-3p,miR-NC,Mock were tested by q RT-PCR and Western blotting.4.Stable overexpression of miR-1-3p cells were transfected with miR-1-3p inhibitor,and the expression of miR-1-3p and its target gene were tested by q RT-PCR and Western blotting.5.Dual-luciferase reporter assay?.miR-1-3p suppresses cell proliferation,the clone formation experiment,scratches,Transwell experiment,apoptosis and tumor formation in nude mice via downregulating PD-L1 expression.1.Hepatoma cell and stable overexpression of miR-1-3p hepatoma cell were both transfected with si RNA PD-L1,and the expression of PD-L1 were tested by q RT-PCR and Western blotting.2.stable overexpression of miR-1-3p cells were transfected with miR-1-3p inhibitor,and investigated its effect on the growth of hepatocellular carcinoma cells.Then continued to transfection si RNA PD-L1 silence the expression of PD-L1,observed the cell proliferation,the clone formation experiment,scratches,Transwell experiment,apoptosis and tumor formation in nude mice.Results:?.miR-1-3p suppresses the expression of PD-L11.miR-1-3p was successful infected cells,transfection efficiency of miR-1-3p in Hep3 B was raised to 93.3%,Hep G2 was raised to 91.5% by flow cytometry.2.RNA-hybridization and Dual-luciferase reporter assay found PD-L1 was potential downstream targets of miR-1-3p.3.The expression of PD-L1 was decreased after overexpression of miR-1-3p.4.The expression of PD-L1 was increased after suppression expression of miR-1-3p.5.Dual-luciferase reporter assay found PD-L1 was potential downstream targets of miR-1-3p.?.miR-1-3p suppresses tumor biological function by targeting PD-L1.1.si RNA PD-L1 silence the expression of PD-L1 effectively.2.miR-1-3p inhibitor inhibited hepatoma cells proliferation,colony formation,migration,invasion,promoted apoptosis and restrain tumor formation in nude mice by targeting PD-L1.And transfection miR-1-3p inhibitor to stable overexpression of miR-1-3p hepatoma cell,observed it promoted hepatoma cell proliferation,cloning,migration,invasion,nude mice into tumor and inhibit cell apoptosis;we continued transfection si RNA to stable overexpression of miR-1-3p hepatoma cell with miR-1-3p inhibitor for silence the expression of PD-l1,observed it inhibits cell proliferation,the cloning,scratches,and nude mice into tumor invasion,promoted the apoptosis of hepatoma cell.Conclusions:miR-1-3p suppresses tumor biological function though targeting PD-L1 in hepatocellular carcinoma.