| Objective:Yiqi Huayu Jiedu decoction(YHJD)combined with chemotherapy in patients with stage II-III gastric cancer after operation.Analysis the effect of treatment,quality of life and prognosis of patients.Exploring YHJD’s vitro effcts on the epithelial-mesenchymal transition and apoptosis of human gastric cancer cell line MGC-803 and the vivo effects on the subcutaneous tumor in nude mice,and to explore its possible mechanism of inhibiting the recurrence and metastasis of gastric cancer.Methods:1.A total of 87 patients with gastric cancer,42 patients in the control group(chemotherapy alone group)and 45 patients in the treatment group(Yiqi Huayu Jiedu Decoction + Chemotherapy)were collected through the medical record system.Compare the curative effect of TCM syndromes,quality of life,tumor marker,Overall Survival(OS)and Progression Free Survival(PFS)of two groups.2.MTT assay,Cell Adhesion Assay,Wound-Healing Assay and Transwell Assay were used to detect the effect of YHJD on the proliferation,adhesion,migration and invasion ability of gastric cancer cell line MGC-803.The EMT-related proteins were detected by Western Blot Analysis.3.The effects of YHJD on apoptosis,cell cycle and apoptosis-related proteins of gastric cancer cell line MGC-803 were detected by Flow Cytometry,Cell Cycle Assay and Western Blot Analysis.4.The gastric cancer cell line MGC-803 was used to establish the subcutaneous tumor model in nude mice.The daily activities,mental status and tumor growth of nude mice were observed during the administration.The inhibition rate of tumor volume and weight were calculated.Changes of EMT-related proteins in xenograft tumors were detected by Western Blot Analysis and Immunohistochemistry Assay.The serum level of TGF-P,IL-6,IL-8 and IL-10 in nude mice were detected by ELISA assay.Result:1.After 2 cycles of treatment,the treatment group had significant improvement on the four symptoms of fatigue,stomachache,pantothenic acid and vomiting,with significant difference compared with the control group(P<0.05).Compared with the control group,the treatment group can significantly improve the overall health status and emotional function of patients(P<0.05).Compared the two groups,the overall survival of the treatment group was significantly prolonged(P<0.05),and the difference was statistically significant(P<0.05).2.YHJD can inhibit MGC-803 cells’ proliferation,adhesion,migration and invasion ability.Western Blot Analysis show that the expression of E-cad increased and the expression of N-cadherin,Vimentin,TGF-β,Snail decreased after treated with YHJD in MGC-803 cells.3.Yiqi Huayu Jiedu can induce the apoptosis of gastric cancer cells,arresting cells in the G0/G1 phase.Western Blot Analysis showed that the expression of Bax,Cleaved Caspase-3 and PTEN increased and the expression of Bcl-2,PI3K,AKT and PDK1 decreased after treated with YHJD in MGC-803 cells.4.YHJD can effectively improve the general condition of nude mice.The volume and weight of xenograft tumor of YHJD group were significantly less than that of the control group(P<0.01).Western Blot Analysis and Immunohistochemistry Assay showed that compared with the control group,the expression of E-cadherin increased and the expression of N-cadherin,Vinmentin,Transforming Growth Factor β(TGF-β),TGF-βR I,p-Smad2,p-smad3,Twist,Snail and Slug decreased after treated with YHJD in nude mice.YHJD can decrease the serum level of TGF-p,IL-6 and IL-8Conclusion:1.YHJD can improve quality of life and prolong overall survival of postoperative patients with gastric cancer.2.YHJD can obviously inhibit the epithelial-mesenchymal transition of human gastric cancer cell MGC-803,induce gastric cancer cell apoptosis,and inhibit the growth of xenograft tumor in nude mice.3.YHJD can inhibit the epithelial-mesenchymal transition of human gastric cancer cell line MGC-803 through TGF-β/Smad pathway.4.YHJD can promote the apoptosis of human gastric cancer cell line MGC-803 through the PI3K/AKT pathway. |
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