CDKN3,ACTG2,TROAP And TSTA3 Network Construction And Analysis In HCC

Single functional molecular network construction and analysis of disease is very useful to identify novel markers and potential targets for prognosis and therapy.We first identified the significant molecule CDKN3,ACTG2,TROAP and TSTA3,then constructed CDKN3,ACTG2,TROAP and TSTA3 up-and down-stream network by gene regulatory network infer(GRNInfer)and further data-mined the main CDKN3,ACTG2,TROAP and TSTA3 modules from 25 human hepatocellular carcinoma(HCC)and 25 no-tumor hepatitis/cirrhotic liver tissues from HCC patients in the same GEO Dataset GSE10140-10141 by using the database for annotation,visualization and integrated discovery(DAVID).CDKN3 modules included biopolymer modification,cell cycle process,biopolymer metabolic process and disease mutation.Our computation showed the different gene rate of CDKN3 network in HCC as 55%(21/38)considering activation and inhibition relationship.We obtained the positive results of CDKN3 biopolymer modification,cell cycle process and biopolymer metabolic process through the net numbers of activation minus inhibition compared with the control and predicted the increases of these modules in HCC,whereas the negative result of CDKN3 disease mutation,and deduced the decrease of this module in HCC.ACTG2 modules included contained nucleotide-binding,organelle,sequence variant and cytoskeleton.Our infer ACTG2 network result showed the different gene rate of HCC as 41%(16/39)compared with the control considering activation and inhibition relationship.Our integrative analysis showed the positive results of ACTG2 nucleotide-binding,organelle,sequence variant and cytoskeleton through the net numbers of activation minus inhibition compared with the control and predicted the increases of these modules in HCC.TROAP modules included phosphoprotein and cellular process Our computation showed the different gene rate of TROAP network in HCC as 48%(24/50)considering activation and inhibition relationship.We gained the negative results of TROAP phosphoprotein and cellular process through the net numbers of activation minus inhibition compared with the control and predicted the decreases of these modules in HCC.TSTA3 modules contained protein modification process,catalytic activity and metabolic process.Our computation showed the different gene rate of TSTA3 network in HCC as 62%(28/45)compared with the control considering activation and inhibition relationship.We obtained the negative results of TSTA3 protein modification process,catalytic activity and metabolic process through the net numbers of activation minus inhibition compared with the control and predicted the decreases of these modules in HCC.