Topography Regulates The Behavior And Effects Of MADSCs

Topological structure of biomaterials has a one-to-many correspondence relationship with multiple cell differentiation.Currently,most studies showed that mechanotransduction plays an important role in the interaction between stem cells and biomaterials;however,there is no systematic study that has been conducted on the transcriptomic level.In this study,we cultured mouse adipose-derived stem cells(mADSCs)on biomaterials with either aligned or random surface topography to study the effect of topography on stem cell differentiation.Various cell staining methods and qPCR revealed that aligned materials induced mADSCs toward tendogenic lineages,while random materials promoted smADSCs to differentiate into osteoblasts,chondrocytes and adipocytes.A whole transcriptome expression analysis,combined with next generation sequencing,was also performed to verify this phenomenon and uncover the potential mechanism of it.CFU and multipotent differentiation detection showed that mADSCs cultured on aligned materials possessed superior sternness potential when compared to random materials.Results further illustrated that metabolic regulation also played a crucial part in d:irecting stem cell's fate.So the metabolic switch might have occurred in mADSCs when they were seeded on materials with random surface topography,decreasing their sternness characteristics.This explanation was indirectly confirmed by performing histone acetylation IF and qPCR to detect the related genes;detection of sternness-related genes was also performed,providing additional direct evidence that the sternness property of mADSCs decreased after culturing on randomly-oriented materials.These results would be helpful in the future to develop novel biomaterials for specific lineage differentiation.Moreover,this study w:ill pave the way for more mechanism studies to explain the effect of physical properties of biomaterials on cells.