| Background TBI occurs suddenly,the disease is changeable and dangerous,the mechanism is more complex,the prognosis is difficult.Although the TBI diagnosis and observation in addition to the CT,MRI and other imaging examination,and injury severity score(ISS)and acute physiology and chronic health evaluation(APACHE)system scores,but it cannot accurately assess the clinical observation and radiographic indicator TBI,more difficult to judge prognosis.Intracranial pressure,blood pressure,blood oxygen and blood glucose levels can only be used as monitoring indicators for temporary conditions,which are often delayed and may lead to delays in optimal treatment.Therefore,one of the most important subjects in the study of craniocerebral trauma is to look for simple and easy observation indicators to improve TBI's observation,treatment guidance and prognosis.In recent years,it has been found that inflammatory response is the main factor of pathological changes in brain injury,and the abnormal level of serum C3 bR and Il-6 is closely related to the degree of traumatic brain injury.This study investigated the relationship between serum C3 bR and il-6 levels in patients with traumatic craniocerebral injury,and the relationship between them and traumatic craniocerebral injury.Objective Observation of patients with traumatic craniocerebral injury C3 bR in serum and the change of IL-6,traumatic C3 bR after craniocerebral injury and to explore the changes of IL-6 mechanism of joint detection in patients with traumatic craniocerebral injury C3 bR and IL-6 in serum of clinical significance.Methods Randomly selected 48 patients with traumatic craniocerebral trauma treated at the 171 Central Hospital of PLA from May 2016 to May 2017 as the observation group,including 26 males and 22 females,aged 25 to 60 years,and average age(43.7±5.8years old.Glasgow coma scale method(GCS)was used according to the patient's injury:?7 points in 18 cases and ?8 points in 30 cases.In the same period,30 healthy people who had physical examinations in our hospital were selected as the control group,and the physical examination results were normal.Among them,there were 16 males and 14 females,aged 23 to 58 years and mean age(41.2±6.3)years old.There was no significant difference in the general data of age,weight,gender,etc.between the groups(P>0.05),and they were comparable.The levels of serum C3 bR and IL-6 were measured by enzyme-linked immunosorbent assay(ELISA)on the first,third,and seventh day of admission in each group,and the expression of C3 bR,IL-6 and the two combined tests were analyzed.All the patients collected 5 ml of fasting venous blood in the early morning on the 1st,3rd,and 7th days of hospitalization.In the same period,5 ml of venous blood was collected in the fasting control group.The specimens were centrifuged at 3000r/min for 10 min.The supernatant was collected and stored in a sealed-70°C environment for testing.Enzyme-linked immunosorbent assay(ELISA)was used to jointly monitor the serum levels of C3 bR and IL-6 and carefully follow the instructions.Sample reagents of C3 bR and IL-6 were purchased from Shenzhen Ereli Biotechnology Co,Ltd.In actual operation,in addition to selecting better reagents,they should be rigorously implemented in accordance with the manual steps,and quality control should be performed at the same time.When testing samples,immediately centrifuge the sample and select 1 mL of the supernatant liquid into an autoclaved 1.5 mL EP tube.Result The level of serum C3 bR on the first,third and seventh days after traumatic brain injury was significantly lower than that of the control group,and the degree of IL-6after traumatic brain injury was significantly higher than that of the control group(P<0.05).In the observation group,patients with light and medium GCS scores ?8 were compared with those with heavy GCS scores?7.Patients with ?8 scores had significantly higher C3 bR levels than ?7,while the former had significantly lower IL-6 levels than the latter.Significance(P<0.05).Compared with those with a heavy GCS score of ?7,thosewith moderate and light GCS scores of ?8 in this group had significantly higher C3 bR levels than the latter(P<0.05).The results suggest that the more severe the traumatic brain injury,the deeper the coma,the more obvious the decrease of C3 bR level and the worse the prognosis.The results of serum IL-6 levels in patients with varying degrees of traumatic craniocerebral injury are shown in Table 3.In this group,the patients with lighter GCS scores ?8 and those with heavy GCS scores ?7 were significantly lower than the former.The difference was statistically significant(P<0.05).The results suggest that the more severe the traumatic brain injury,the deeper the coma,the more obvious increase of IL-6level and the worse the prognosis.The combined detection of serum C3 bR,IL-6 and ROC curve area were 0.624,0.641and0.900,respectively.The analysis showed that both C3 bR and IL-6 were more sensitive to detection,and had higher sensitivity than single detection in the same period,and the ROC curve was lower.The largest area.It shows that the combined detection of serum C3 bR and IL-6 can significantly improve the sensitivity of diagnosis of traumatic brain injury.Conclusion Serum C3 bR and IL-6 combined detection can significantly improve the sensitivity of traumatic brain injury diagnosis,so as to understand the severity of traumatic brain injury,will help to assess the disease and determine the prognosis,in clinical practice has important Clinical value. |
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