The Impact Of Different Concentrations Of EGCG On The Antibacterial And Physical Properties Of Glass Ionomer Cement

ObjectiveTo evaluate the antibacterial and physical properties of glass ionomer cement(GIC)after the addition of different concentrations of epigallocatech in-3-gallate(EGCG),and to gain the best integrative concentration of EGCG.MethodsBacteria S.mutans UA159 was used in this study.The EGCG-GIC was prepared by mixing 0%,0.05%,0.1%,0.15%,0.2% and 0.25% concentration of EGCG(w/w)in GIC.The bacterial inhibition zone of the agar-diffusion assessment and OD 570 value in the MTT test were quantified to testify the antibacterial activity of materials from different groups.The effect of different concentrations of EGCG on the mechanical properties of materials was comprehensively evaluated: including the compressive strength by electronic universal testing machine,the flexural strength determined by ISO standard,and the surface microhardness tested using microhardness tester.ResultsEGCG effectively improved the antibacterial properties of GIC,and the antibacterial properties increased with the increase of EGCG concentration(P<0.05).The mechanical properties(compressive strength,flexural strength,and surface microhardness)of GIC gradually strengthened with the increase of EGCG and reached the maximum when the add ratio was 0.15%,then decreased with the increase of EGCG(P <0.05).ConclusionsGIC supplemented with EGCG has good antibacterial activity against the cariogenic S.mutans UA159.As the content of the added antibacterial agent increases,the antibacterial performance will continue to increase;when the mass ratio of the EGCG antibacterial agent added to the GIC is 0.15%,the mechanical properties of the composite material is best.In summary,EGCG with a mass ratio of 0.15% can maximize the antibacterial and mechanical strength of GIC at the same time.