Nanofiber Structures And Surface Modification Of Ti6Al4V Based On Ultrasonic Surface Rolling Processing And Plasma Nitriding For Enhanced Bone Regeneration

Artificial bone repair materials and guide bone regeneration materials play important roles in bone grafting recent years.Nowadays,it is very crucial in orthopedics research for seeking and creating great guide bone regeneration materials that have better biological characteristics.In order to spread thinking in the area of bone defect repair and regeneration,biocompatibility and the ability of osteogenesis of PCL/Gelatin nanofiber and surface modification Ti6Al4 V treated by Ultrasonic Surface Rolling Processing(USRP)and Plasma Nitriding(PN)was investigated in this study.Here polycaprolactone(PCL,polymer)/gelatin(protein)hybrid nanofibrous GBR membranes were fabricated via electrospinning,followed by crosslinking with genipin.Meanwhile,Ti6Al4 V modified using ultrasonic surface rolling processing(USRP)and plasma nitriding(PN)was research for its biocompatibility and the ability of osteogenesis.Using SEM,AFM FTIR,XPS,TGA,DSC XRD and water contact angle for researching material's surface topography and properties.CCK-8,SEM images,ALP and alizarin red tested the biocompatibility and the ability of osteogenesis of materials.The results are as follows:(1)the proportion of PCL and gelatin in the as-spun nanofiber membranes could be simply adjusted by changing the weight ratio of PCL and gelatin in the spinning solution.SEM and AFM images demonstrated that all the nanofibers possessed uniform and smooth structures both in two dimension(2D)and three dimension(3D).The mechanical tests showed that these nanofibers exhibited appropriate tensile and strength properties,which were suitable for bone tissue engineering.CCK-8 and SEM images revealed that all the membranes were biocompatible to MC3T3-e1 cells.In addition,the in vitro osteogenesis characterizations,alizarin red in normal medium and osteogenesis medium,indicated that the nanofibers could promote bone formation.Therefore,all these results could suggest that our design of electrospun polymer/protein nanofiber membranes was effective for guided bone regeneration.(2)USRP1+PN showed the highest surface roughness(268 ?m).The water contact angle measurements demonstrated that USRP2 had more favorite wettability(75.7°).The XRD analysis indicated the enhancement of nanocrystallization for the samples treated by USRP and the formation of a nitride layer for the samples treated by USRP and PN.The interactions between the Ti6Al4 V samples and MC3T3-E1 cells were investigated in vitro by SEM,CCK-8 test,alkaline phosphatase(ALP)activity assay,and Alizarin Red S staining.The SEM images and CCK-8 tests revealed that all the Ti6Al4 V samples were biocompatible to MC3T3-E1 cells,and the results of ALP activity assay and Alizarin Red S staining showed a better osteogenesis performance for the samples treated by USRP and PN.The present study indicates that USRP and PN may act as an effective method for Ti6Al4 V to induce bone regeneration.