| Objective: This study is based on a new calcium sulfate/silk fibroin dual-delivery composite material combined with traditional Chinese medicine,and mainly assessed its efficiency both in controlling infection and repairing the bone defect through the in vitro antibacterial,sustained-release-drug experiment and in vivo animal experiments.Methods:First,the antibacterial properties and sustained-release effect of the composite material were detected in vitro.Second,we established the femur osteomyelitis model of New Zealand rabbit by Methicillin-Resistant Staphylococcus Aureus(MRSA).After debrided,the rabbits were randomly divided into three groups:A:without the composite material and routinely fed;B:with the composite material and routinely fed;C:with the composite material and fed by traditional Chinese medicine.The result of antiinfection and osteogenesis is assessed by the methods of X radio-imageology,micro-CT and histopathology.Results: The inhibition zone was formed in Staphylococcus aureus bacterium incubation dish with the composite material for 16 hours of incubation.The concentration of antibiotics remains high due to the sustained-release system.The results of X-ray,Micro-CT and histopathology showed that there was no infection and lots of newly formed bone trabecula in transplanted site.Conclusion:The use of this new calcium sulfate/silk fibroin dual-delivery composite material combined with traditional Chinese medicine can be a potential therapeutic agent for infected bone defects due to its concomitant osteoinductive and antibiotic properties. |
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