Influence And Significance Of Hydrogen Sulfide On The Expression Of Acute Lung Injury's GRP78 Of Rats Which Is Induced By Paraquat

Objectives:To observe the changes of GRP78 and TNF-?in acute lung injury induced by paraquat poisoning,to investigate the influence and significance of hydrogen sulfide on the expression of acute lung injury's endoplasmic reticulum stress protein GRP78of rats which is induced by paraquat..MethodsExperiment 1:50 S-D rats were randomly divided into 10 groups,including paraquat poisoning group?3h,6h,12h,24h,48h?,normal saline control group?3h,6h,12h,24h,48h?.Observation general view of lung tissue and pathological changes of lung tissue under light microscope,blood gas analysis of abdominal aorta,determination of protein content in bronchoalveolar lavage fluid by BCA,lung wet/dry weight ratio,determination of TNF-?in peripheral blood by ELISA,determination of endogenous H₂S in serum by spectrophotometry,GRP78mRNA content in lung tissue was detected by fluorescence quantitative PCR,detection of GRP78 expression in lung tissue by Western Blot.20 S-D rats were randomly divided into 4 groups:blank control group?C group?,paraquat poisoning normal saline group?N group?,exogenous H₂S group?H group?,H₂S inhibitor group?P group?.Observation general view of lung tissue and pathological changes of lung tissue under light microscope,blood gas analysis of abdominal aorta,determination of protein content in bronchoalveolar lavage fluid by BCA,lung wet/dry weight ratio,determination of TNF-?in peripheral blood by ELISA,GRP78mRNA content in lung tissue was detected by fluorescence quantitative PCR,detection of GRP78 expression in lung tissue by Western Blot.ResultsExperimentation 11.Morphological and pathological changes of lung1.1 Gross macroscopic viewThe lung tissue of normal saline control group was pale pink,the structure was normal,the capsule was complete and smooth,the texture was soft,the surface was normal,and the elasticity was good.The lung tissue of paraquat poisoning group with dark red,hyperaemia,surface smooth,hard texture,surface bleeding,ecchymosis,lung tissue fluid,bleeding,bleeding,blisters,poor elasticity,is most obvious in the48h group.1.2 HE staining under microscopeIn normal saline control group,the lung tissue structure was complete,the alveolar wall was complete,the alveolar space was clear,and there was no congestion,edema and exudation in the pulmonary interstitium.Inparaquat poisoning group,the damage of alveolar structure,alveolar inflammatory cells,red blood cell invasion;interstitial lung congestion,edema,exudation,pulmonary interstitial capillary dilatation,hyperemia,micro thrombosis,hyaline membrane formation,is most obvious in the 48h group.2.Changes in blood gas analysisThe PaO₂,PaCO₂,SaO₂value of arterial blood in the normal saline control group were in the normal range;The PaO₂,PaCO₂,SaO₂value of paraquat poisoning group at each time point were significantly lower than that of normal saline control group,the difference was statistically significant?P<0.05??3.Changes of lung wet dry/weight ratio and BALF protein concentrationCompared with normal saline control group at each time point of paraquat poisoning group,the lung wet dry/weight ratio and BALF protein concentration of paraquat poisoning group was significantly higher than that of the normal saline control group,the difference was statistically significant?P<0.05??4.Changes of serum TNF-?and endogenous H₂S contentComparison of paraquat poisoning group and normal saline control group at different time points,the TNF-?level in serum of rats exposed to paraquat was increased,and the content of endogenous H₂S decreased,the difference was statistically significant?P<0.05?.5.Changes of GRP78mRNA content and GRP78 expressionComparison of paraquat poisoning group and normal saline control group at different time points,the content of GRP78mRNA and the expression of GRP78 in lung tissue of rats exposed to paraquat was significantly increased,the difference was statistically significant?P<0.05?.6.Analysis of the correlation between the content of endogenous H₂S and the content of GRP78mRNA and the expression of GRP78 and serum TNF-?content,analysis of the correlation between the serum TNF-?contentand content of GRP78mRNA and the expression of GRP78The content of endogenous H₂S in serum of rats exposed to paraquat was negatively correlated with the content of GRP78mRNA in lung tissue and the expression of GRP78 protein in lung tissue and serum TNF-?content?P<0.01?;The content of TNF-?in serum of rats exposed to paraquat was positively correlated with the content of GRP78mRNA and the expression of GRP78 protein in lung tissue?P<0.01?.Experimentation 21.Morphological and pathological changes of lung1.1 Gross macroscopic viewThe lung tissue of C group was pale pink,the structure was normal,the film was smooth,the texture was soft,the surface was normal,and the elasticity was good;N group,H group and P group rats lung tissue was dark red,hyperaemia,surface smooth,hard texture,surface see petechia,ecchymosis,lung tissue fluid,bleeding,bleeding,blisters,poor elasticity,the degree of severity was P>N>H.1.2HE staining under microscopeIn the C group,the lung tissue structure was complete,the alveolar wall was complete,and the alveolar space was clear.the N group,H group,P group,the alveolar structure destroy,alveolar inflammatory cells,red blood cell invasion;interstitial lung congestion,edema,exudation,pulmonary interstitial capillary dilatation,serious congestion,micro thrombosis,hyaline membrane formation,the degree of severity was P>N>H.2.Changes in blood gas analysisThe PaO₂,PaCO₂,SaO₂value of arterial blood in C group were in the normal range;in group N,group H and group P,there were different degrees of hypoxemia,hyperventilation,decreased oxygen saturation and acidosis,the degree of severity was P>N>H,the difference was statistically significant?P<0.05??3.Changes of lung wet dry/weight ratio?BALF protein concentration and serum TNF-?levelsN group,P group,H group compared with C group,lung wet dry weight ratio?W/D??BALF protein concentration and serum TNF-?levels increased in different degrees,the degree of severity was P>N>H,the difference was statistically significant?P<0.05??4.Changes of GRP78mRNA content and GRP78 expressionN group,P group,H group compared with C group,the content of GRP78mRNA and the expression of GRP78 protein increased in different degrees,the degree of severity was P>N>H,the difference was statistically significant?P<0.05??Conclusions1.After paraquat poisoning,the content of endogenous H₂S decreased,the expression of GRP78 in lung tissue was increased,and the level of serum TNF-?was increased2.Exogenous H2S reduced the expression of GRP78 in lung tissue,decreased the level of serum TNF-?,and reduced the degree of lung injury.