Detection Of ?-synuclein By Minor Salivary Gland Biopsy In The Diagnosis Of Parkinson's Disease And Multiple System Atrophy

Objectives:Todetect the expression of alpha-synuclein(?-Syn)in the two groups of patients with Parkinson's disease(PD),multiple system atrophy(MSA)and healthy controls by minor salivary gland biopsy.To further explore the value of ?-Syn for the diagnosis of PD,the differential diagnosis between PD and MSA.Methods:We enrolled 18 patients from the Department of Neurology from February 2015 to April 2017 in the Second Affiliated Hospital of Soochow University,including 12 clinically diagnosed PD patients,6 clinically probable MSA patients,and 6 age and gender matched healthy controls.All participants underwent a general information collection.Minor salivary gland biopsy was performed in all subjects by the samestomatologistaccording tothe standard procedure,and the expression of ?-Syn was detected by immunohistochemistry using ?-Syn antibody.Results: Of the 12 PD patients,9 had positive immunohistochemical staining.The positive rate of ?-Syn was 75%.4 of the 6 MSA patients had positive immunohistochemical staining,the positive rate was 66.67%.No positive immunohistochemical staining of ?-Syn was observed in all healthy controls.Conclusions:The detection of ?-Syn in minor salivary gland biopsy may be an early diagnostic marker of PD,with high sensitivity and specificity.The detection of ?-Syn may not be a biomarker for distinguishing PD and MSA now,it still needs a large sample of research.